BCH5413 TESTED QUESTIONS WITH
REVISED ANSWERS
Type of information found in a cDNA library - Answer-The genes that are expressed in a
particular tissue or cell type. cDNA is reverse transcribed from the RNA that was
expressed in a tissue or cell type.
the purpose of electromobility shift assay... - Answer-determine if a specific protein
binds to a specific DNA in vitro
If a protein of interest binds to a labeled oligonucleotide, it will increase the molecular
weight of the fragment and shift it to a higher position in an acrylamide gel.
the goal of ChIP-Seq is to identify... - Answer-All DNA sequences bound to a protein of
interest
in nonsense mutation - Answer-a single base change creates a stop codon instead of
an aa
the advantage of Cassette Mutagenesis over QuikChange... - Answer-in Cassette
Mutagenesis no PCR is necessary, so there is no possibility of replication errors.
in the example of stable transfection of sFRP1 in human kidney cancer cells, match
each technique to the rationale for including the technique to the study: microarray,
cloning into a shuttle vector, stable transfection, western blot, measure tumor volume in
mouse model - Answer--microarray: identify the genes that are overexpressed in kidney
cancer cells. sFRP1 was one of those genes
-cloning into a shuttle vector: create a sFRP1 construct that can be maintained in E.coli
and mammalian cells
-stable transfection: create a cell line that permanently express sFRP1
-western blot: determine that the sFRP1 protein is overexpressed from a plasmid
construct on a stable cell line
-mouse model: determine if the overexpression of sFRP1 gene has an affect on tumor
volume
Advantages of GST fusion protein system... - Answer--there is no competition with
bacterial proteins (bacterial cells do not contain GST)
-prevent inclusion bodies (GST fusion can increase solubility of a protein that you are
trying to express)
Which of the following statements best describes a heterozygous gene locus?
a. The two chromosomes have different alleles at the locus
b. One of the two chromosomes has a mutation at the locus
c. The two chromosomes have the same alleles at the locus
REVISED ANSWERS
Type of information found in a cDNA library - Answer-The genes that are expressed in a
particular tissue or cell type. cDNA is reverse transcribed from the RNA that was
expressed in a tissue or cell type.
the purpose of electromobility shift assay... - Answer-determine if a specific protein
binds to a specific DNA in vitro
If a protein of interest binds to a labeled oligonucleotide, it will increase the molecular
weight of the fragment and shift it to a higher position in an acrylamide gel.
the goal of ChIP-Seq is to identify... - Answer-All DNA sequences bound to a protein of
interest
in nonsense mutation - Answer-a single base change creates a stop codon instead of
an aa
the advantage of Cassette Mutagenesis over QuikChange... - Answer-in Cassette
Mutagenesis no PCR is necessary, so there is no possibility of replication errors.
in the example of stable transfection of sFRP1 in human kidney cancer cells, match
each technique to the rationale for including the technique to the study: microarray,
cloning into a shuttle vector, stable transfection, western blot, measure tumor volume in
mouse model - Answer--microarray: identify the genes that are overexpressed in kidney
cancer cells. sFRP1 was one of those genes
-cloning into a shuttle vector: create a sFRP1 construct that can be maintained in E.coli
and mammalian cells
-stable transfection: create a cell line that permanently express sFRP1
-western blot: determine that the sFRP1 protein is overexpressed from a plasmid
construct on a stable cell line
-mouse model: determine if the overexpression of sFRP1 gene has an affect on tumor
volume
Advantages of GST fusion protein system... - Answer--there is no competition with
bacterial proteins (bacterial cells do not contain GST)
-prevent inclusion bodies (GST fusion can increase solubility of a protein that you are
trying to express)
Which of the following statements best describes a heterozygous gene locus?
a. The two chromosomes have different alleles at the locus
b. One of the two chromosomes has a mutation at the locus
c. The two chromosomes have the same alleles at the locus
