MB (ASCP) TEST ATCUAL EXAM 300 QUESTIONS AND CORRECT (100% VERIFIED ANSWERS)
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Predominant form of DNA -Correct Answer Right-handed B
DNA and histone interaction is due to what type of bond? -Correct Answer Ionic
What stains to the minor groove of DNA duplex? -Correct Answer SYBR Green
What is the most abundant type of RNA? -Correct Answer Ribosomal RNA (rRNA)
Wha...
MB (ASCP) TEST 2023-2024 ATCUAL
EXAM 300 QUESTIONS AND CORRECT
(100% VERIFIED ANSWERS)
Predominant form of DNA -Correct Answer ✔Right-handed B
DNA and histone interaction is due to what type of bond? -Correct Answer ✔Ionic
What stains to the minor groove of DNA duplex? -Correct Answer ✔SYBR Green
What is the most abundant type of RNA? -Correct Answer ✔Ribosomal RNA (rRNA)
What does RNA act as in a retrovirus? -Correct Answer ✔Genome and mRNA
What increases half-life of mRNA? -Correct Answer ✔5' Methyl Cap
What kind of DNA is most likely to be transcriptionally active? -Correct Answer
✔Euchromatin
What is the most common type of eukaryotic DNA? -Correct Answer ✔Satellite DNA
What specimen has the most DNA according to molecular weight? -Correct Answer
✔White Blood Cells
What amino acids compose histones? -Correct Answer ✔Lysine and Arginine
Amino acid that all proteins start with: -Correct Answer ✔Methionine
What protects the DNA strand during replication? -Correct Answer ✔Single-stranded
binding protein
Leading Strand -Correct Answer ✔3' to 5' template
Lagging Strand -Correct Answer ✔5' to 3' template
What DNA strand is being copied in transcription? -Correct Answer ✔Antisense Strand
First step after newly formed mRNA is made? -Correct Answer ✔Addition of poly(A) tail
MB (ASCP) EXAM 2023-2024 ATCUAL EXAM
, MB (ASCP) EXAM 2023-2024 ATCUAL EXAM
Every time the chromosome is copied it would become shorter if not for what enzyme? -
Correct Answer ✔Telomerase
What polymerase has repair activity? -Correct Answer ✔Beta/Epsilon
What polymerase acts on leading strand? -Correct Answer ✔Delta
What polymerase acts on lagging strand? -Correct Answer ✔Alpha
Which polymerase is used for mitochondrial replication and repair? -Correct Answer
✔Gamma
What polymerase binds the 5' end to 3' end of dsDNA? -Correct Answer
✔Topoisomerase
Which RNA polymerase is mainly for transcription? -Correct Answer ✔RNA Pol II
Which polymerase is involved with mRNA synthesis? -Correct Answer ✔RNA Pol II
What inactivates RNAses? -Correct Answer ✔Diethyl pyrocarbonate (DEPC) or bake
glassware for 4 to 6 hours at 400C
What type of restriction enzyme recognizes palindromic sites? -Correct Answer
✔Restriction Enzyme Type II
What type of restriction enzyme lacks methylation activity? -Correct Answer
✔Restriction Enzyme Type II
What is necessary to make cDNA? -Correct Answer ✔Reverse transcriptase
Number of SNPs in a human genome? -Correct Answer ✔11 billion (10^7)
How many genes (base pairs) are in a human haploid genome? -Correct Answer ✔3
billion base pairs
The polymerization of acrylamide requires: -Correct Answer ✔Catalyst (APS or TEMED)
or Light activation
How does agarose gel pore size relate to concentration? -Correct Answer ✔Inversely
proportional
What is denatured during a southern blot? -Correct Answer ✔Hydrogen bonds (inter-
strand bonds)
MB (ASCP) EXAM 2023-2024 ATCUAL EXAM
, MB (ASCP) EXAM 2023-2024 ATCUAL EXAM
Melting temperature formula -Correct Answer ✔Tm = (4C x GC Pairs) + (2C x AT pairs)
Which is likely to interfere with fluorescent probe? -Correct Answer ✔5' G
Proper GC content of primer -Correct Answer ✔35-65%
Inhibitors of PCR -Correct Answer ✔Hemoglobin, Heparin, Xylene, Phenol, Cyanol, and
Bromophenol Blue
Order to add to a PCR reaction: -Correct Answer ✔Master mix > patient sample >
positive control > negative control
Pre Area -Correct Answer ✔Positive Pressure
Post Area -Correct Answer ✔Negative Pressure
Which PCR requires two steps for specificity? -Correct Answer ✔Nested PCR
Which microorganism is detected by branched DNA amplification? -Correct Answer
✔DNA or RNA
Which technique uses RNA, antibodies, and DNA? -Correct Answer ✔Hybrid Capture
Assay
Use of bisulfite treatment of DNA to determine its pattern of methylation -Correct
Answer ✔Bisulfite DNA
What tests are isothermal? -Correct Answer ✔NASBA, TMA, & SDA
Which leukemia is associated with FLT3 gene? -Correct Answer ✔AML
T-cell gene rearrangement is seen in what malignancy? -Correct Answer ✔Sezary
Syndrome
Which translocation causes Acute Premyelocytic Leukemia? -Correct Answer ✔t 15;17
Ewing Sarcoma -Correct Answer ✔t 11;22
Synovial Sarcoma -Correct Answer ✔t X;18
Follicular Lymphoma -Correct Answer ✔t 14;18
MB (ASCP) EXAM 2023-2024 ATCUAL EXAM
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