Expression cloning - Samenvattingen, Notities en Examens

Prescott's Microbiology, 11e (Willey) Chapter 1 The Evolution of Microorganisms and Microbiology 1) Extant microorganisms are organisms from the fossil record that are no longer present on Earth today. Answer: FALSE Topic: Taxonomy of Microorganisms Bloom's/Accessibility: 2. Understand / Keyboard Navigation ASM Topic: Module 01 Evolution ASM Objective: 01.05 The evolutionary relatedness of organisms is best reflected in phylogenetic trees. Learning Outcome: 01.02a Propose a timeline of the ori...

BCH5413 TEST 2 QUESTIONS AND ANSWERS ALL REVISED AND UPDATED Methylation: a primitive immune system for bacteria - hds-R - Answer-- plasmid transforms into cells are protected (restriction enzyme not expressed) Methylation: a primitive immune system for bacteria - mcrA/mcrB/mrr - Answer-degrades foreign DNA that is not properly methylated - can occur to DNA obtained from mouse/human cells containing CpG methylated DNA (can interfere with cloning of mammalian genomic DNA) pCR 2.1 - TOP...

RNAS-C Exam 4 Practice Test 75 Questions with Verified Answers A couple expecting their second child is worried about Rh incompatibility. Their first child was Rh+. They have their blood typed. Which result would be cause for alarm? A) both parents Rh- B) both parents Rh+ C) man Rh- and woman Rh+ D) man Rh+ and woman Rh- - CORRECT ANSWER D) man Rh+ and woman Rh- Research indicates that allergies may actually be due to a mutation in the gene that encodes a skin protein called __________...

Topic 1 Introduction to Molecular Cloning 1. The goal of this integrated practical course is to purify a protein. In general, what is the value of protein purification to medical research? What information can a purified protein provide and what techniques can be developed/employed once you have a purified protein?  In vitro studies - Small molecule interactions, protein binding partners etc  Producing antibodies  Determination of structure Purification of the protein is a valua...

What is genetic engineering? - using in vitro techniques to alter genetic material in the lab What is recombinant DNA technology? - the artificial recombination of DNA from two organisms What are some basic techniques of genetic engineering? - DNA amplification electrophoesis nucleic acid hybridization molecular cloning expressing foreign genes targeted mutagenesis What is heterologous expression? - expressing a gene in a different host What is the polymerase chain reaction? - rapid a...

Bio 202 EXAM 3 QUESTIONS AND CORRECT DETAILED ANSWERS How is recombinant DNA technology used to clone the human GH gene? 1st step! - ANSWER 1. use PCR to obtain the gene (Simply cutting the DNA with restriction enzymes would not be an effective way to isolate a specific gene. The genome would be cut into so many fragments, if you ran a gel it would be a smear! Which fragment is yours? You would need hybridization to isolate your fragment. Wouldn't have much DNA- not like the amo...

Describe DNA structure - Answer-DNA forms a double stranded helix and adenine pairs with thymine, and cytosine pairs with guanine What pair forms hydrogen bonds and how many - Answer-thymine/adenine form 2 bonds guanine/cytosine form 3 bonds Explain "the central dogma" - Answer-DNA turning into RNA turning into Protein What is transcription - Answer-Copying of DNA code to RNA, one nucleotide at a time so it is complementary to the DNA What is translation - Answer-RNA gets translated int...

UCLA Anthro 124 Final Exam Questions With Verified Correct Solutions What is the logic of evolutionary psychology Computational Theory of Mind? - ANS Brain viewed as a computational computer that has functions that uses information gathered in a functional way What is it known as if we know consciously that it is harmless and not gross? We still won't accept it - ANS encapsulation and automaticity of disgust What three questions does sexual reproduction arise? - ANS 1) why...

What is the purpose of having rrnBT4 in an expression vector? - correct answerEnhances the stability of the GOI transcript Why must reverse transcriptase be used to create a eukaryotic expression library? - correct answerThe resulting cDNAs do not contain non-protein coding introns Which type of ligation-independent cloning technique takes advantage of the recombination between lambda phage attB and attP sequences in the vectors and in the DNA fragment to be inserted? - correct answerGatew...

EPPN 154 Exam 3 Questions & Answers 2024/2025 Expressing foreign genes in bacteria - ANSWERSTranscription and translation to produce coded proteins Obstacles in expressing foreign genes in bacteria - ANSWERS1. Genes placed under control of a bacterial promoter 2. Introns must be removed 3. Codons might need editing 4. Many eukaryotic proteins require host modification after translation Expression Vectors allow for control of what? - ANSWERSexpression of the cloned genes How mus...