Rt pcr - Samenvattingen, Notities en Examens

MCB 2050 Molecular Biology of the Cell - W21 Practice Midterm exam questions and answers tips with Dr. Yankulov University of Guelph PART A 1. Which of the following is NOT an essential component of a cloning vector? a. A unique restriction endonuclease cleavage b. A dominant selectable marker gene c. An origin of replication d. A Taq polymerase binding site 2. Methylation of DNA sequence is used how by bacteria? a. Methylation of DNA can inhibit cell division b. Methyla...

Among its many other uses, PCR is finding important applications in early clinical diagnosis of disease. An example is in testing for the presence of viruses to which antibodies do not appear until a long time after infection. Describe how PCR could be used to detect HIV infection at an early stage, including what information about HIV would be needed to begin with. PCR could be used to detected HIV by performing PCR on cells with primers that are designed to amplify the viral HIV DNA that...

WGU Biochemistry OA (Latest 2022/2023) Verified Answers Bohr Effect chart (low pH) C- High CO2 H- High H+ A- Acidic R-Release of O2(rt. Shift) T- tense state (in tissues) Primary structure Sequence of amino acids that make up a protein Secondary Structure Sequence of amino acids fold. Tertiary Structure -more folding in a 3D shape. -R groups (side chains) Quaternary Structure Protein has more than 1 polypeptide chain. Chemical reaction -absorb and release en...

Most common STD - CORRECT ANSWER Chlamydia (1.7 mil cases) Skin normal biota is coagulase-negative staph, cornybacterium, and microbacterium smegmatis - CORRECT ANSWER males (all ages) Skin normal biota staph and corynbacterium - CORRECT ANSWER females (prepuberty and post-menopause) Normal biota of the skin for reproductive-age females in some cases- they carry it asymptomatically - CORRECT ANSWER GBS Normal biota of the skin for ___________ includes Enterobacteriace...

Creatine levels in older and younger adults: are levels different? Ans: aging adults have lower Pcr levels compared to younger between old and young; which one does creatine benefit more? Ans: young which muscle fiber(s) carry creatine? Ans: TYPE II will HIT exercise maintain type 2 fibers and creatine storage? Ans: yes true or false: aging does not have a negative effect on high energy phosphate and why we see results in older and younger people. Ans: true 2 why might older adults ...

ASCP MB: Extra-Practice Questions and Answers Scored A The Klenow fragment of E. coli DNA polymerase I is used: - Answer ️️ -to label DNA probes by random primer labeling. What is a solution hybridization method for the detection of nucleic acid:protein interactions? - Answer ️️ -gel mobility shift assay _____ involves mixing a labeled oligonucleotide with a protein extract to see if a specific interaction occurs, visible with an apparent increase in molecular size on a gel due to...

Acute and Episodic Exam 1 - Complete Solutions (Answered) A patient who has undergone surgical immobilization for a femur fracture reports dyspnea and chest pain associated with inspiration. The patient has a heart rate of 120 beats per minute. Which diagnostic test will confirm the presence of a pulmonary embolism? D-dimer CT angiography Arterial blood gases Electrocardiogram A 65-year-old patient who has not had an influenza vaccine is exposed to influenza and comes to the clinic the followi...

PA Family Medicine EOR Questions and Answers 100% correct Deteriorating mental status/unconscious patient ABC's IOM's Airway security Breathing Circulation IV access O2 saturation Monitor vitals Acute Respiratory Distress Syndrome ↓ PaO2 and ↑ PaCO2 from fluid collapsing the alveoli MCC: 6-72 hrs s/p Sepsis Associated w/ Renal & Hepatic failure ↓ BP or shock w/ crackles, rales, wheezing Tx: Mechanical Vent w/ PEEP Anaphylaxis (Type 1 Hypersensitivity) Upper...

MB (ASCP) 2024 - Translocations, Diseases, Pharma, Polymerases, & more Chronic Myeloid Leukemia (CML) - Answer ️️ -t(9;22) BCR-ABL translocation Ewing sarcoma - Answer ️️ -t(11;22) EWS-FLI1 Acute Lymphocytic Leukemia (ALL) - Answer ️️ -t(9;22) BCR-ABL p190 protein Follicular Lymphoma - Answer ️️ -t(14;18) BCL2 translocation Mantle Cell Lymphoma - Answer ️️ -t(11;14) bcl-1 translocation CCND1-IgH Burkitt Lymphoma - Answer ️️ -t(8;14) MYC gene translocation R...

EPPN 154 Exam 3 Questions & Answers 2024/2025 Expressing foreign genes in bacteria - ANSWERSTranscription and translation to produce coded proteins Obstacles in expressing foreign genes in bacteria - ANSWERS1. Genes placed under control of a bacterial promoter 2. Introns must be removed 3. Codons might need editing 4. Many eukaryotic proteins require host modification after translation Expression Vectors allow for control of what? - ANSWERSexpression of the cloned genes How mus...