UAMS MICROBIOLOGY CASE DEVELOPMENT
Study Set 2 questions with complete solutions
Perform 2 different ID tests CORRECT ANSWER Procedure for medicolegal
cases when an isolate appears to be GC
Isolate specific GNR such as salmonella and shigella CORRECT ANSWER
Purpose of GN broth
Probable UTI CORRECT ANSWER One organism with a colony count of 10^5
CFU/mL
Probable Contaminant CORRECT ANSWER Multiple organisms with equal
colony counts
Probable UTI CORRECT ANSWER one organism with a colony count of 10^5
CFU/mL and one organism with 30 colonies
Number of colonies x calibration factor of loop
(0.1mL = .01mL= 1000) CORRECT ANSWER formula for calculating
colony forming units
Vaginal cells with bacteria growing on them CORRECT ANSWER Clue cells
Bacterial Vaginosis CORRECT ANSWER Disease associated with clue cells
Gram negative-Gram variable CORRECT ANSWER organisms associated with
bacterial vaginosis
,Superficial skin contamination CORRECT ANSWER presence of epithelial cells
on a gram stain could indicate what?
Clinically Significant CORRECT ANSWER Organisms in smears prepared from
high quality specimens are probably what?
Normal flora CORRECT ANSWER Viridans Strep, Strep Pneumo, CNS, Neisseria
Sp, Haemophilius Sp, E. coli, Bacillus sp, BHSB, Enterococci, Yeast, Lactobacilli
and Clostridium sp can be considered what? (Normal Flora or pathogens)
from infected animals CORRECT ANSWER How is Brucella and Pasturella
transmitted?
From contaminated water CORRECT ANSWER How is Aeromonas and
Legionella Transmistted?
From Sexual contact CORRECT ANSWER How is Chlamydia Transmitted?
Metallic Green Sheen CORRECT ANSWER E. coli appearance on EMB
Pink mucoid CORRECT ANSWER Klebsiella on MAC
Colorless CORRECT ANSWER Salmonella and Shigella on EMB, MAC, SS
agars
Salmonella CORRECT ANSWER Between salmonella and shigella, which one is
H2S positive?
, Fermenting Lactose CORRECT ANSWER Why does E. coli, Klebsiella and
Enterobacter appear pink on MAC, Red on SS, Yellow-orange on HE and yellow
on XLD?
Catalase +
Coagulate +
Beta Hemolysis
GPC in clusters CORRECT ANSWER Key Biochemical tests of S. aureus
Catalase +
Coagulase -
Novobiocin Resistant
GPC clusters CORRECT ANSWER Key biochemical tests of S. saprophyticus
Catalase+
Coagulase -
GPC clusters CORRECT ANSWER Key biochemical tests of CNS
Oxidase +
Bacitracin Sensitive
Furazolidone Resistant
GPC in tetrads CORRECT ANSWER Key biochemical tests of Micrococcus
Bacitracin Sensitive
SXT resistant
Study Set 2 questions with complete solutions
Perform 2 different ID tests CORRECT ANSWER Procedure for medicolegal
cases when an isolate appears to be GC
Isolate specific GNR such as salmonella and shigella CORRECT ANSWER
Purpose of GN broth
Probable UTI CORRECT ANSWER One organism with a colony count of 10^5
CFU/mL
Probable Contaminant CORRECT ANSWER Multiple organisms with equal
colony counts
Probable UTI CORRECT ANSWER one organism with a colony count of 10^5
CFU/mL and one organism with 30 colonies
Number of colonies x calibration factor of loop
(0.1mL = .01mL= 1000) CORRECT ANSWER formula for calculating
colony forming units
Vaginal cells with bacteria growing on them CORRECT ANSWER Clue cells
Bacterial Vaginosis CORRECT ANSWER Disease associated with clue cells
Gram negative-Gram variable CORRECT ANSWER organisms associated with
bacterial vaginosis
,Superficial skin contamination CORRECT ANSWER presence of epithelial cells
on a gram stain could indicate what?
Clinically Significant CORRECT ANSWER Organisms in smears prepared from
high quality specimens are probably what?
Normal flora CORRECT ANSWER Viridans Strep, Strep Pneumo, CNS, Neisseria
Sp, Haemophilius Sp, E. coli, Bacillus sp, BHSB, Enterococci, Yeast, Lactobacilli
and Clostridium sp can be considered what? (Normal Flora or pathogens)
from infected animals CORRECT ANSWER How is Brucella and Pasturella
transmitted?
From contaminated water CORRECT ANSWER How is Aeromonas and
Legionella Transmistted?
From Sexual contact CORRECT ANSWER How is Chlamydia Transmitted?
Metallic Green Sheen CORRECT ANSWER E. coli appearance on EMB
Pink mucoid CORRECT ANSWER Klebsiella on MAC
Colorless CORRECT ANSWER Salmonella and Shigella on EMB, MAC, SS
agars
Salmonella CORRECT ANSWER Between salmonella and shigella, which one is
H2S positive?
, Fermenting Lactose CORRECT ANSWER Why does E. coli, Klebsiella and
Enterobacter appear pink on MAC, Red on SS, Yellow-orange on HE and yellow
on XLD?
Catalase +
Coagulate +
Beta Hemolysis
GPC in clusters CORRECT ANSWER Key Biochemical tests of S. aureus
Catalase +
Coagulase -
Novobiocin Resistant
GPC clusters CORRECT ANSWER Key biochemical tests of S. saprophyticus
Catalase+
Coagulase -
GPC clusters CORRECT ANSWER Key biochemical tests of CNS
Oxidase +
Bacitracin Sensitive
Furazolidone Resistant
GPC in tetrads CORRECT ANSWER Key biochemical tests of Micrococcus
Bacitracin Sensitive
SXT resistant
