BIO 198L Midterm Practical Exam Questions and Correct Answers

Electromagnetic (EM) spectrum visible light electromagnetic energy is classified based on the wavelength highest energy= gamma radiation the spectrophotometer is used to measure the specific wavelengths and the quantity of light absorbed by a substance the extent to which a solution absorbs light is called the absorbance higher absorbance value equals darker solution the absorbance of a solution is found by using Beer's Law, A=Ebc a measure of how well the substance absorbs light at that frequency extinction coefficient distance light travels through a solution (cm) path length standard curve is a graph showing the relationship between the amount of substance and it's absorbtion the purpose of the blank in the spectrophotometer is to calibrate the spectrophotometer the purpose of spectrophotometer is to measure the amount of light through a solution Bradford assay interacts with R-group of specific amino acids The greater the concentration of the amino acids in a solution the deeper the color primary structure is the sequence of amino acids that make up polypeptides and determine how it folds/its function secondary structure is the sequence is which the shapes are within the polypeptide 3-dimensional structure of an entire polypeptide tertiary structure