HTL
University Of Mississippi Medical Center
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HTL (Histotechnologist) Bundle Set
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HTL (Histotechnologist) Bundle Set
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HTL – Carbohydrates questions with correct answers
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Glycogen is most abundant in - Answer Liver, heart (cardiac muscle cells), womb 
 
Staining reactions for glycogen: - Answer PAS (+), diastase (sensitive), Bauer-feulgen (+), Best carmine (+) 
 
Which two glycogen fixatives preserves the most glycogen? - Answer Bouins & Rossman's 
 
Other glycogen fixatives - Answer 80% alcohol, alcoholic formalin (37% formalin, 95% alcohol), acetic alcohol formalin, aqueous solution (formalin) 
 
This type of fixative should be avoided for glycogen fix...
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HTL Histotechnology: Special Stains questions with correct answers
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This is a stain for acid mucopolysaccharides and sialomucins. - Answer Alcian blue 
 
This stains reacts with cations and is used to find calcium in tissue. - Answer Alizaran Red S 
 
This stains uses ferric ions to demonstrate mucopolysaccharides and glycoproteins. Must be done at a low pH. - Answer Colloidal iron 
 
In this stain, tissue is first treated with alkali to open up binding sites through the release of hydrogen bonds. - Answer Congo Red 
 
This stain,combined with HCl and pa...
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HTL BOC STUDY GUIDE Part 1 questions with correct answers
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Cryptococcus neoformans - Answer a fungus that can be demonstrated with various stains for acidic mucins is 
 
the P.H. should be modified on the 4.6 stain - Answer ATPase stains done on a muscle biopsy at P.H.9.4,4.6 & 4.3.The slides for P.H.9.4 & 4.3 show good differentiation of the type 1 & 2,& the slide done at P.H.4.6 shows differentiation of type 1 & 2 fibers,it doesn't show differentiation of the type 2A and 2B fibers.This indicates that the 
 
hemochromatosis - Answer a patholo...
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HTL Immunohistochemistry questions with correct answers
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used to determine the origin of a tumor, prognosis and treatment. - Answer Immunohistochemistry 
 
is a host protein (immunoglobulin) produced in response to the presence of foreign molecules, organisms, or other agent in the body. - Answer antibody 
 
Immunoglobulins are proteins produced by ________________ in response to antigenic stimulation. - Answer B lymphocytes 
 
determines the Ig subclasses (IgG, IgA, IgE, IgD, IgM) - Answer heavy chains 
 
the site at which the antibody at...
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HTL Prep questions with correct answers
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The primary purpose of fixation is - Answer To stop enzymatic action 
 
Which fixative contains picric acid, formalin, and acetic acid? - Answer Bouin 
 
The volume of fixative should exceed the volume of the tissue by - Answer 15-20 times 
 
Tissue must be washed in water after fixation in - Answer Zenkers 
 
Which fixative contains formalin, potassium dichromate, and mercuric chloride? - Answer Helly 
 
Precipitate left in tissues that have been fixed in solutions containing mercuric...
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