Bios 242 week 2 lab - Study guides, Class notes & Summaries

Lab12:BacteriaoftheRespiratorySystem Introduction: Therespiratorysystemisdividedintotwoparts:theupperandlowerrespiratorysystems.Thelowerrespiratorysystemisanaxenicenvironmentbecauseof the cilia that lines the respiratory tract, alveolar macrophages and IgA antibodies found in the mucus. The upper part harbors a variety of microbiotasuch as Gram-negative bacteria Hemophilus, Gram-positive bacteria such as Staphylococcus aureus and diphtheroids that can cause opportunisticinfectionsleading toinf...

BIOS 242 Week 1 Assignment; Lab 2 of 14 Onsite - Isolation of Pure Cultures Lab 2: Techniques for Isolation of Pure Cultures Learning Objectives: Isolate pure cultures from a mixed population of microorganisms using aseptic technique. Learn the streak plate method Introduction: Microorganisms exist in nature as mixed populations of different species. In order to study them and observe their characteristics it is first necessary to obtain pure cultures (a culture ...

Lab3:Microscopy-ObservationofCells LearningObjectives: • Usethecompoundlightmicroscopeforobservationofcells. • Identifydifferenttypesofbacteriabasedontheirshapes. • Identifyavarietyofeukaryoticcells. Introduction: Exercise2:ObservationofDifferentTypesofBacteriaMaterials: Compoundmicroscope,immersionoil,lenswipes,preparedslidesofvariousbacteria-Rods,cocciandspiral Method: 1. Obtainthepreservedslideofabacteria. 2. UseAppendixAandfollowtheinstructionsonhowtouseaMicroscope. 3. O...

BIOS 242 Week 2 Assignment; Lab 3 of 14 Onsite; Microscopy Observation of Cells Lab 3: Microscopy- Observation of Cells Learning Objectives: Use the compound light microscope for observation of cells. Identify different types of bacteria based on their shapes. Identify a variety of eukaryotic cells. Introduction: Microbiology is the study of organisms, which cannot be seen with naked eye. You will need help of a microscope to observe these “tiny animalcules” as they w...

Lab 4: Simple Staining Learning Objectives: • To learn about principle of staining • To learn the process of simple stain • To prepare wet mount slides Exercise 1: Simple Stain Materials: Overnight cultures of S. epidermidis, B. subtilis, S. cerevisiae (yeast) , inoculating loop, glass slides, incinerators, crystal violet or any other stain chosen by instructor; tongs, staining racks/trays, immersion oil, lens paper, bibulous paper, microscope Method: 1. Obtain a clean glass slid...

BIOS-242: | BIOS 242 FUNDAMENTALS OF MICROBIOLOGY WITH LAB WEEK 2 QUESTIONS WITH ANSWERS

Lab 3: Microscopy- Observation of Cells Learning Objectives: • Use the compound light microscope for observation of cells. • Identify different types of bacteria based on their shapes. • Identify a variety of eukaryotic cells. Introduction: Exercise 2: Observation of Different Types of Bacteria Materials: Compound microscope, immersion oil, lens wipes, prepared slides of various bacteria- Rods, cocci and spiral Method: 1. Obtain the preserved slide of a bacteria. 2. Use Appen...

BIOS 242 Week 2 Assignment; Lab 4 of 14 Onsite; Simple Staining Lab 4: Simple Staining Learning Objectives: To learn about principle of staining To learn the process of simple stain To prepare wet mount slides In light microscopy, colored dyes, called stains are used to improve contrast between the specimen and the background surrounding the specimen. Staining makes visualization of the specimen easier due to increased contrast. This is crucial for specimens that are mostl...

BIOS 242 Week 3 Assignment; Lab 5 of 14 Onsite; Differential Staining Learning Objectives: Explain the principle of differential staining Apply differential staining to identify bacteria Differential stain uses two or more stains to specifically stain certain structures or cellular components which cannot be easily observed using simple stains. Differential staining principles are based upon the specific chemical nature and composition of cellular components and therefore, different s...

BIOS 242 Week 4 Assignment; Lab 7 of 14 Onsite; Disinfectants and Antibiotics Lab Report: Disinfectants and Antibiotics Purpose: Describe the purpose of the lab. Exercise 1: Disinfectants Organism 1: Be sure to record the chemical or product you tested and complete this table by obtaining data from other groups in your class Organism 2: Be sure to record the chemical or product you tested and complete this table by obtaining data from other groups in your class Q...