Blotting - Study guides, Class notes & Summaries

Western blotting a. Proteins from SDS-PAGE are transferred to a nitrocellulose membrane using electrical current a. membrane is blocked so antibody will not bind nonspecifically b. Primary antibody is added and incubated with the membrane c. To detect antibodies that have bound to protein a secondary anti-immunoglobulin antibody coupled to a reporter a. Excess secondary antibody is washed off the membrane b. Substrate is added that precipitates upon reaction c. Reveals visible band where...

NR 228 Nutrition study guide exam with questions and answers Vitamin K - co factor in synthesis of blood clotting factors including prothrombin. Protein formation in bone, kidney, and plasma also need Vitamin K

MCAT - Lab Techniques (MilesDown)Native-PAGE - correct answer A polyacrylamide gel electrophoresis method for proteins using NON-DENATURING conditions. Proteins keep their native charge and structure so they are separated based on charge and size. SDS-PAGE - correct answer A polyacrylamide gel electrophoresis method for proteins using DENATURING conditions. Sodium Dodecyl Sulfate denatures the proteins and gives the proteins a uniform charge. This allows them to be separated solely on mass, t...

A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as A. southern blotting B. northern blotting C. polymerase chain reaction D. DNA replication

MCAT - Lab Techniques (MilesDown) Native-PAGE - correct answer A polyacrylamide gel electrophoresis method for proteins using NON-DENATURING conditions. Proteins keep their native charge and structure so they are separated based on charge and size. SDS-PAGE - correct answer A polyacrylamide gel electrophoresis method for proteins using DENATURING conditions. Sodium Dodecyl Sulfate denatures the proteins and gives the proteins a uniform charge. This allows them to be separated solely on mass,...

Craniofacial Biology, Exam 1 with Practice Questions and Answers Name two specific purposes for cloning foreign DNA into plasmid vectors ANSWER protein drug production (like insulin) and gene therapy Briefly describe the cloning process ANSWER -restriction endonuclease digests and inserts the DNA into a vector with ligation - introduce newly ligated DNA into host cell (like E coli) -identify bacteria that have taken up the proper + vector Where to RE come from and what do Restriction ...

BCHM102 Midterm Exam Practice Questions and Answers aminoacyl tRNA synthetase requires ______ energy - Correct Answer️️ -ATP translocation requires ________ energy - Correct Answer️️ -GTP two methods of translation termination - Correct Answer️️ -hairpin formation and rho protein post-transcriptional modification - Correct Answer️️ -the modifications that occur to an mRNA transcript before it is exported from the nucleus as mature mRNA. Include: splicing of introns, addit...

DAT Mnemonics Practice Exam Questions & Answers. Cell Cycle - CORRECT ANSWER Go Sam Go Make Cake G1 growth phase 1 of interphase S synthesis of interphase G2 growth phase 2 of interphase M mitosis of M phase C cytokinesis of M phase Nitrogenous bases - CORRECT ANSWER PUR As Gold = purines = adenine and guanine CUT the PY = pyrimidines = cytosine, uracil, thymine Types of blots - CORRECT ANSWER SNOW DROP southern blotting = DNA northern blotting = RNA western blotting ...

Which wound would be allowed to heal by secondary intention? A. Cleft lip repair B. Infected hysterectomy incision C. Exploratory laparoscopy incision D. Facial laceration caused by a pocket knife Ans--B. Infected hysterectomy incision Which action would minimize the risk for cross-contamination while cleansing an infected abdominal surgical wound? A. Cleansing the wound with sterile water B. Blotting the incision with dry gauze C. Wearing sterile gloves to cleanse the wound D. Using...

Bio 205 Practice Guide Assessment Questions with Best Graded Marking Scheme 2024 Why is a nitrocellulose membrane used in western blotting? - correct answer stronger and can be used for other processes How do proteins move from the polyacrylamide gel onto nitrocellulose? - correct answer apply negative electrodes on gel side and positive on membrane side and flow contents from negative to positive What is the third stage of western blotting? - correct answer probing and hybridization W...