Bright field microscopy - Study guides, Class notes & Summaries

Resolution Correct Answer-small dist b/t 2 pts can be distinguished; D = .61λ/Nsinα, want lower D; Light microscope 200nM Bright field microscopy Correct Answer-normal light type Phase-contrast Correct Answer-altered phase interference of unobstructed light; looks like cells are surrounded by light DIC Correct Answer-3D looking cells Confocal Correct Answer-3D imaging with fluorescence, progessively capture full surface in focus by removing out of focus; normal point-scanning vs....

Bright field The most commonly used type of microscopy is called _____, because the specimens produce dark images against brighter backgrounds. Dark field In ______ microscopy, however, bright images on a darker background are produced because only light that is refracted by the specimen is captured. Phase contrast The _____ microscope uses slight differences in refractive indices and an annular condenser to produce sharper images than simple bright-field microscopy. Differential interference...

carl woese correct answers what scientist discovered the archael domain? 16 sRNA gene correct answers what gene sequence did Woese use to determine that archaea were evolutionary different from bacteria? false correct answers T/F: archaea are only found in extreme environments Asgard archaea correct answers what group of archaea are eukaryotes most closely related to? a hydrothermal vent system correct answers what environment was the first Asgard archaeon discovered in? electrica...

Microbiology Lab Midterm Questions and Answers Already Passed Compound microscope 1. Ocular-eyepiece lens (usually 10x) 2. Objective-nosepiece lenses (commonly 4x, 10x, 45x, and 100x) At what magnification do you use the oil immersion technique? 100x. Total Magnification = Magnification (Objective) x Magnification (Ocular) Important Parts of the Microscope Framework, Stage, and Lens System Framework Arm and base are the structural parts of the microscope which support the basic frame. Stage...

beta-oxidation correct answer: enzymes repeatedly split off pairs of they hydrogenated carbon atoms that make up a fatty acid and join each pair to coenzyme A to form acetyl-CoA Chloroplasts correct answer: light harvesting structures found in photosynthetic eukaryotes; synthesize a few polypeptides compare and contrast archaeal flagella with bacterial flagella correct answer: archaeal are about half as thick as bacteria; not hollow; lack a central channel; flow together; powered by ATP...

Electron beams can not be used to resolve images of objects. T/F? Correct Answer-False In all types of electron microscopy, electrons pass through the specimen. Correct Answer-False This type of light microscopy can be used to view very thin cells or flagella. It does that by using this property of light. Correct Answer-Dark field; Light scattering In this type of microscopy, light is delayed by passing through the sample and so objects look as if they are out of phase. It employs this ...

MIBO 3500 Exam 1 Latest Update Graded A+ millimeter (mm) 10^-3 micrometer (um) 10^-6 nanometer 10^-9 Microbes Organisms and acellular agents too small to be seen by the unaided eye Robert Hooke made first compound microscope, coined the term "cell" Antoine van Leeuwenhoek built single-lens magnifier, first to observe single-felled microbes Francesco Redi meat and maggots research, disproved theory that microbes spontaneously generated Lazzaro Spallanzani also disproved the spontaneous g...

BMSC 210 Midterm 1 Study Guide | 100% Correct Answers | Verified | Latest 2024 Version What are properties of all cells? - Growth Metabolism Evolution LUCA - Last universal common ancestor How do we decide how the phylogenetic tree is drawn? - Use ribosomes. All organisms have it and it has been the same for billions of years What are the domains? - Bacteria Archaea Eukarya What defines the domains of cells? - Their rRNA sequencing What domains are most closely related?...

Major differences between plant and animal cells correct answers Animal: Only cell membrane Plastids Absent (heterotrophic) Vacuole One or few small Shape Irregular, no mechanical support In vitro regeneration: Single cell can not regenerate into a new organism Lysosomes Present Plant: Cell wall is present outside cell membrane, made of cellulose Plastids Present, perform photosynthesis (autotrophic) Large vacuole for storage (about 90%) of cell space in mature cells Fixed shape...

micrometer CORRECT ANSWER one millionth of a meter nanometer CORRECT ANSWER one billionth of a meter two critical factors that influence our ability to see an object CORRECT ANSWER resolution and contrast resolution CORRECT ANSWER the distance between two objects at which the objects still can be seen as separate contrast CORRECT ANSWER the difference in light absorbance between two (objects) the closer two objects are to each other the __________________ the resolution requiremen...