Histotechnology - Study guides, Class notes & Summaries

Processing includes (no fixation) CORRECT ANSWER dehydration, clearing, and infiltration. Dehydration CORRECT ANSWER the removal of water, a process necessary to prepare the tissue for embedding in a nonaqueous medium such as paraffin, celloidin, and some plastics. Excessive dehydration CORRECT ANSWER when bound water may be removed in a nonadditive reaction. This results in hard and brittle tissue that is difficult to section. Incomplete dehydration CORRECT ANSWER of tissues during pro...

fixitive used for specimens - correct answers 10% neutral buffered Formalin Marking Margins - correct answers - to identify resection margins, if tumour too close to these margins they know they have to go in and cut more out - Guide to embedding What is used to clean grossing station - correct answers phenolic disenfectant or gluteroldehyde how long is specimens stored for - correct answers 1-3 months some legal cases kept indefinitly Why is fresh tissue not stored? - correct answ...

Select the procedure suitable for demonstrating secretory granules in a carcinoid tumor: A. Wilder reticulum B. PAS-aniline blue C. Snook D. Sevier-Munger D. Sevier-Munger The Sevier-Munger technique is an excellent argyrophil procedure for demonstrating carcinoid tumors. It is similar to, but shorter than, the Grimelius. In Bodian's method for nerve fibers and neurofibrils, Protargol impregnates both neurofribrils and connective tissue. Subsequently, connective tissue is rendere...

Histotechnology Practicum: Test 1Biopsy or Gross Specimen: small piece of tissue and derived from simple-natured procedure - correct answers biopsy biopsy or Gross specimen: whole organ and typically derived from surgeries - correct answers gross specimen What are the 10 steps in the pathology lab workflow? - correct answers 1. fixation 2. accession 3. grossing 4. continue fixation 5. dehydration 6. clearing 7. infiltration 8. blocking 9. section 10. staining what is the most i...

Histotechnology test 2What is the consequence to improper clearing? - correct answers "Moth eaten" due to water still being in the tissues- improper dehydration Sections will crumble and fall out of block Shrink the centre of the tissue Why would a tissue shrink? - correct answers Wax is too hot What causes microscopic chatter? - correct answers Over dehydration seen most prominately at the ends of tissues What is the melting point do most paraffins? - correct answers 55-58 degr...

What thickness are paraffin sections routinely cut? CORRECT ANSWER-4-6 um Why should the temperature of the flotation bath be set specifically at 5-10 °C below the melting point of the paraffin wax? CORRECT ANSWER-water bath that is too hot may distort tissue morphology Warm enough to relieve compression Cool enough so the wax does not melt What thickness are paraffin sections cut for the Luxol Fast Blue procedure? CORRECT ANSWER-10 - 15 um Why are paraffin blocks put on ice pr...

Place in a separate container CORRECT ANSWER-Match each listed item with the appropriate disposal method: Used silver nitrate Simply pour down the sink CORRECT ANSWER-Match each listed item with the appropriate disposal method: 1% alcohol Dilute first, pour down the sink CORRECT ANSWER-Match each listed item with the appropriate disposal method: Concentrated HCL Place in a biohazard bag CORRECT ANSWER-Match each listed item with the appropriate disposal method: Blood-stained...

What are the two common staining methods for nucleic acid in histology? CORRECT ANSWER-1. Feulgen Reaction 2. Methyl Green-Pyronin Method (DNA/RNA) Feulgen Reaction tests for what? CORRECT ANSWER-DNA What does the Schiff reagent bind to in the Feulgen Reaction? CORRECT ANSWER-Aldehyde group created by the hydrolysis of DNA Why can't we use the Bouin fixative on tissues being tested with Feulgen? CORRECT ANSWER-Bouin hydrolyzes tissues In Feulgen reactions, what happens when the hyd...

Histotechnology Final 1st Quarter T/F To be characterized as a profession, considerable specialized training must be required. - correct answers T Histology is the study of - correct answers tissue T/F The purpose of having a procedure for specimen receipt is to monitor the specimen and the accompanying paperwork. - correct answers T T/F Accessioning is giving a specimen a common number, one that is not unique to the patient. - correct answers f T/F The tasks performed by the histot...

Histotechnology--Nuclear & Cytoplasmic Stainingc. nuclei *hemalum stains (hematoxylin mordanted with either ammonium aluminum sulfate or potassium aluminum sulfate) are used almost exclusively to stain nuclei Harris hematoxylin is used on tissue sections to stain: a. fat b. glycogen c. nuclei d. cytoplasm b. oxidation *can be done naturally (atmospheric oxygen), or with oxidizing agents Ripening of hematoxylin is a process of: a. hydrolysis b. oxidation c. mordanting d. ...