Inoculum - Study guides, Class notes & Summaries

BIOS 242 Week 1 Assignment; Lab 2 of 14 Onsite - Isolation of Pure Cultures Lab 2: Techniques for Isolation of Pure Cultures Learning Objectives: Isolate pure cultures from a mixed population of microorganisms using aseptic technique. Learn the streak plate method Introduction: Microorganisms exist in nature as mixed populations of different species. In order to study them and observe their characteristics it is first necessary to obtain pure cultures (a culture ...

BIOL 2460 Chapter 9: Microbial Growth Graded A+ Most common form of bacterial reproduction Binary fission 4 Basic Steps of Binary Fission 1. Growth of cell size and increase in cell components 2. Replication of DNA/chromosomes 3. Division of cytoplasm (cytokinesis) 4. Septum formation and division of daughter cells Cytokinesis is directed by the ___________ protein. FtsZ Where is the Z ring assembled during binary fission? On the cytoplasmic membrane Divisome activates the...

What is the single most important way to reduce the spread of unwanted microbes? handwashing Where and how should used media (culture tubes and plates) be disposed of? directly into bio hazard bin Where and how should a slide prepared by you (ex: Gram stain) be disposed of? Wrapped in a paper towel and disposed into the biohazard bin Where should gloves worn by you (after lab, not cleaning up a spill) be disposed of? in the regular trash can Where should a broken stock slide (ex: 3 forms sli...

Binary Fission : Most common form of bacterial reproduction Binary Fission 4Basic Steps : 1.Growth of cell size and increase in cell components 2.Replication of DNA 3.Division of the cytoplasm (cytokinesis) 4.Septum formation and division of daughter cells Z ring Assembly : •Cytokinesis is directed by FtsZ protein •FtsZ assembles Z ring to form divisome •Divisome activates production of peptidoglycan and septum Generation Time : •Generation Time (Doubling Time) - time takes ...

Lab 2: Techniques for Isolation of Pure Cultures Learning Objectives:  Isolate pure cultures from a mixed population of microorganisms using aseptic technique.  Learn the streak plate method VIDEO:  Making a streak Plate (Bio-Rad Laboratories) Introduction: Microorganisms exist in nature as mixed populations of different species. In order to study them and observe their characteristics it is first necessary to obtain pure cultures (a culture containing only one species of an o...

Binary Fission Most common form of bacterial reproduction Binary fission 4 Basic steps 1. Growth of cell size and increase in cell components 2. Replication of DNA 3. Division of Cytoplasm (cytokinesis) 4. Septum formation and division of daughter cells Fstz 1. Cytokinesis is directed by this protein 2. Assembles Z ring to form (Divisome) What activates the production of a peptidoglycan and septum? Divisome Generation (Doubling Time) Time takes to double population through one round of binar...

CAP is the ___________ infectious ds in the US and the ______________ leading cause of death. - Most deadly - 8th What normally prevents the development of lower respiratory tract infections following aspiration of oropharyngeal secretions containing bacteria or inhalation of infected aerosols? Pulmonary defense mechanisms (cough reflex, mucociliary clearance system, immune responses) CAP occurs when _________________ . There is a defect in 1 or more of the normal defense mechanisms (cou...

4 Basic Steps of binary fission : 1. Growth of cell size 2. Replication of DNA 3. Division of cytoplasm 4. Septum formation & cell division Z Ring Assembly : FtsZ assembles Z ring to form divisome Generation Time : time it takes to double the population Lag Phase : Cells adjust to culture medium; no change in population What factors determine the duration of the lag phase? : Genetic makeup, media composition, initial inoculum size During which phase of the growth curve is it most ap...

4 Basic Steps of binary fission - Answer-1. Growth of cell size 2. Replication of DNA 3. Division of cytoplasm 4. Septum formation & cell division Z Ring Assembly - Answer-FtsZ assembles Z ring to form divisome Generation Time - Answer-time it takes to double the population Lag Phase - Answer-Cells adjust to culture medium; no change in population What factors determine the duration of the lag phase? - Answer-Genetic makeup, media composition, initial inoculum size During which phase of...

CML vs. Leukemoid Reaction - ANSWER CML - decreased leukocyte alkaline phosphatase (LAP) Leukemoid Reaction - increased LAP Look-back - ANSWER The process for the identification and notification of recipients of blood components from prior donations of who now test positive for HCV, HIV-1 and/or HIV-2. External reference electrode used in ISE - ANSWER KCl The serum glucose concentration of a diabetic patient undergoing a 2-hr GTT should return to the baseline (fasting level) after ____...