Steps of pcr - Study guides, Class notes & Summaries

MCB 2050 Midterm W21 – Dr. Yankulov via Respondus Lockdown Browser and Webcam PART A – Questions from tutorial Question 1. You are studying the genes involved in embryonal development. Which of the following techniques could be used to visualize the locations with high gene expression of a particular gene in a whole mounted embryo? a) Western blotting b) Northern blotting c) Southern blotting d) Immunoprecipitation e) RNA in situ hybridization Question 2. Why is Taq DNA polymerase ...

WGU c785 Final Exam 2023 with all the correct answers(Actual test 100% verified) Hemophilia Pedigree - Father has hemophilia, mother does not. What is the outcome for their kids? - CORRECT ANSWER His daughters would be carriers. This is x-link recessive. Autosomal: Dominant: - CORRECT ANSWER Autosomal: males and females equally affected. Dominant: non-carrier parents polymerase chain reaction (PCR) - CORRECT ANSWER The process of copying DNA in the lab. Uses Template DNA, Nucleot...

Final exam Straighter line microbiology 100% SOLUTION 2023//2024 In the nitrogen cycle, which of the following steps involves removal of nitrogen from the atmosphere by bacteria? - ANSWER Nitrogen Fixation If enough H+ ions are added to pure water to raise the H+ concentration of the solution to ten times higher than the original concentration, what is the resulting OHconcentration of the solution? - ANSWER 10-8 M Fungi are able to reproduce asexually by creating conidiospores and spo...

WGU c785 2023 with all the correct answers(Actual test 100% verified) polymerase chain reaction (PCR) - CORRECT ANSWER process to copy DNA What is needed to complete PCR - CORRECT ANSWER Template DNA, Nucleotides (dNTPS), DNA Polymerase, and DNA Steps of PCR - CORRECT ANSWER denaturation, annealing, elongation denaturation - CORRE

BCH5413 TEST 2 QUESTIONS AND ANSWERS ALL REVISED AND UPDATED Methylation: a primitive immune system for bacteria - hds-R - Answer-- plasmid transforms into cells are protected (restriction enzyme not expressed) Methylation: a primitive immune system for bacteria - mcrA/mcrB/mrr - Answer-degrades foreign DNA that is not properly methylated - can occur to DNA obtained from mouse/human cells containing CpG methylated DNA (can interfere with cloning of mammalian genomic DNA) pCR 2.1 - TOP...

BCH 5413 EXAM ONE QUESTIONS WITH VERIFIED ANSWERS If you want to identify the protein PTEN on a Western blot, what is secondary antibody? - Answer-Fab can bind to primary antibody and Fc portion can bind to a HRP antibody so when bound to TMB will light up Which of the following increases the stringency of Southern Blot? Why? - Answer-Increased hybridization, more Interactions between target and probe PCR: primer - Answer-Short ssDNA sequence used in PCR, pair of primers used to hybridize...

Males and Females are equally affected - Autosomal The process of copying DNA in the lab. - Polymerase Chain Reaction (PCR) Template DNA, Nucleotides (dNTPS), DNA Polymerase, and DNA Primers. - What is needed for Polymerase Chain Reaction? Denaturing, Annealing, Elongating - What are the three steps of a Polymerase Chain Reaction (PCR)? Denaturing (DNA is heated up to separate it) - What is the first step of a Polymerase Chain Reaction? Cooling the Polymerase Chain Reaction. Primers stick ...

WGU Biochemistry Final Review 2024 With Complete Solutions Latest Guide A+. 1. The polymerase chain reaction is a tool used to study protein structure. True False - ANSWER-False Feedback PCR is a tool used to amplify a specific segment of DNA. What color is the primer in the following diagram? Red Purple Blue - ANSWER-Red Feedback Recall from the section on DNA replication that DNA polymerase needs a primer to begin DNA synthesis. This requirement means the primers will direct the DN...

During PCR, two DNA strands are separated by breaking hydrogen bonds during the denaturation step T or F True Which of the following is true about PCR? a. only one primer is needed for PCR to proceed b. the purpose of PCR is to amplify a region of DNA c. PCR requires a hot start of 60 c d. PCR melts the covalent bonds between DNA strands b. the purpose of PCR is to amplify a region of DNA Previous Play Next Rewind 10 seconds Move forward 10 seconds Unmute 0:00 / 0:...

WGU Biochemistry OA (Latest 2022/2023) Verified Answers Bohr Effect chart (low pH) C- High CO2 H- High H+ A- Acidic R-Release of O2(rt. Shift) T- tense state (in tissues) Primary structure Sequence of amino acids that make up a protein Secondary Structure Sequence of amino acids fold. Tertiary Structure -more folding in a 3D shape. -R groups (side chains) Quaternary Structure Protein has more than 1 polypeptide chain. Chemical reaction -absorb and release en...