Cloning - Guides d'étude, Notes de cours & Résumés

MCB3020 Exam 3 Questions and Answers 100% CorrectMCB3020 Exam 3 Questions and Answers 100% CorrectMCB3020 Exam 3 Questions and Answers 100% Correct Reverse transcriptase process - ANSWER - 1. poly-T primer to single-strand RNA 2. reverse transcriptase to produce DNA-RNA hybrid 3. enzyme RNaseH to cut RNA and regenerate RNA primers 4, DNA polymerase and ligase synthesizes new complementary DNA strand cloning vector - ANSWER - allows for transfer of gene of interest to a host organism; allo...

1.Consider the statement, "all sick people should see a doctor. An elderly man is ill". therefore, the man A. Should see a doctor B. Should see a doctor if he does not get well C. Is like most ill people D. May not get well unless he sees a doctor 1. Consider the statement, "all sick people should see a doctor. An elderly man is ill". therefore, the man A. Should see a doctor 2.A science student is watching a television debate on the genetic cloning of animals. In order for the stu...

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PRAXIS CRAM - BIOLOGY 5236 LATEST UPDATE GRADED A+ Pros of stem cell research Curing genetic diseases Growing skin for burn victims Growing new organs Cons of stem cell research Harvesting embryonic stem cells kills the embryo, what constitutes a viable embryo? Cons of genetically modified foods Man should not interfere, allergens, modifications not contained-could pass in to wild species Cons of cloning - reduced gene pool - animals less healthy - worries that humans could ...

Consider the statement, "all sick people should see a doctor. An elderly man is ill". therefore the man A. Should see a doctor B. Should see a doctor if he does not get well C. Is like most ill people D. May not get well unless he sees a doctor (Ans- A. Should see a doctor 2.A science student is watching a television debate on the genetic cloning of animals. In order for the student to make a reasoned decision, the student must first A. Justify the opinions presented in the telev...

Biotechnology |Cloning Vectors Questions and Answers

As we discussed before the RAD52 gene can be isolated from the yeast cells. What should we do first so we can isolate the gene we need? - Answer Perform DNA extraction In order to isolate RAD52, what should we do after extracting all DNA from the yeast cells? - Answer Amplify the RAD52 gene There is a plasmid containing eGFP on your workbench. How can we isolate this eGFP gene from the plasmid? - Answer Perform restriction enzyme digestion How can you confirm that we have s...

Snowflake Certification Exam Rated A+ Verified A defined data set can be shared with customers outside the Snowflake account is? - ANSWER-Snowflakes's Data Sharing A defined data set is a point in time snapshot and can be updated by customers is? - ANSWER-Snowflake's Cloning A Snowflake mechanism that is used to limit the number of micro-partitions scanned by a query is called ________? A) Governor B) Regulator C) Pruning D) None of the above - ANSWER-C) Pruning

You want to clone a DNA fragment that has been digested with BamHI into a plasmid vector. What should you do to prevent religation of the vector? If you don't do this what other problems will occur? Correct Answer-To prevent religation you should treat it with alkaline phosphatase which will knock off the 5' phosphate. As a result you will have 5' and 3' OH which means ligase can't act on it. If you don't, you could get a concatemer or the insert could go in upside down or backwards. ...

DNA Cloning and Sequencing Exam Questions and Answers Already Passed why do we clone genes? - Answers it is easier to characterize and manipulate cloned genes. to study genes or gene products. mass produce large quantities of protein. prepare to insert a gene into another organism. what are the 2 ways to make copies of a gene? - Answers insert into vector and replicate in bacteria (in vivo) polymerase chain reaction (in vitro) what do you need to clone bacteria? - Answers vector (plasmid...