Tsi agar slant Study guides, Class notes & Summaries

Types of Tests (4) - ANSWER-TSI Citrate Carbohydrate Fermentation Test w/ Durham Tubes MR/VP TSI Test - ANSWER-Triple Sugar Iron Test (slant) both a streak and a slant Citrate test - ANSWER-typically only a streak is done but both a streak and slant may be performed MR/VP test - ANSWER-Methyl Red Voges- Proskauer TSI Composition - ANSWER-Basic T Soy Agar plus: 1. glucose, lactose, & sucrose. Lac and Suc are 10x the conc. of glucose 2. Phenol red (pH indicator) pH 6...

BIOD 171 lab exams 1-9 LAB 1 Answer the following questions 1. What three elements are used in an autoclave to sterilize equipment? heat, pressure, and steam 2. What is the minimum temp erature an autoclave must be set at to achieve sterile condition? 125°C 3. If you are working in a lab in which an autoclave is not available, and you are pressed for time, which would you chose to best sterilize your equipment? Hot steam or hot air? Explain why you chose your answer. Hot steam is the best choic...

Biochemical Test - Triple Sugar Iron (TSI) Questions with Answers Tests for... - CORRECT ANSWER carbohydrate fermentation, gas production, & hydrogen sulfide production TSI slant - CORRECT ANSWER contains agar, pH indicator (phenol red), 1% lactose, 1% sucrose, 0.1% glucose, & sodium thiosulfate & ferrous sulfate; as media cools after autoclaving, test tubes placed at slanted angle - provides oxygen-rich surface (aerobic) & oxygen-depleted butt (anaerobic) how the test works - CORRECT...

TSI Agar correct answer: *stab and drag *yellow= acid production/ fermentaion of the sugars in the slant/butt. *red= negative *black= H2S production (salmonella or proteus) what are we looking for in the Indole test after adding kovacs reagant? correct answer: * a red ring which indicates that the amino acid tryptophan has been degraded into indole. Methyl red test results? correct answer: red= lots of acid (PH of 4) normally yellow=acidic Simmons citrate correct answer: to see i...

For what bacteria would you use the TSI test? correct answer: Gram negative bacilli such as enterobacteriaceae. How would you identify an acidic slant? correct answer: If the slant goes from red to yellow on the phenol red. Why is there more lactose and sucrose in TSI agar than glucose? correct answer: In order to distinguish bacteria that can only ferment glucose from bacteria that ferment lactose and sucrose. It will also allow for greater acid production. TEST RESULTS: red slant/r...

SALMONELLA NOTES QUESTIONS AND ANSWER 2023 GRADED A+ UPDATE Salmonella ANSWER = Genus with over 2200 pathogenic serotypes Serotype classification ANSWER = Based on Kauffman-White system Gastroenteritis ANSWER = Moderate to severe illness caused by Salmonella Transmission ANSWER = Contaminated water and undercooked food, especially chicken Salmonella typhi ANSWER = Causes typhoid fever Salmonella Subgroup I ANSWER = Includes Salmonella typhi and Salmonella paratyphi Suspect Salmonel...

bio 171 Lab 7 Notebook. Detailed working, solutions, memo, notes & explanations. Lab 7 Notebook Back to Home Page Title: PL#7 Secondary Assays for Bacterial Characterization Objective: This lab identifies the profile of bacterial populations that are based on additional biochemical approaches to best determine the growth and metabolic characteristics. Indole Test: Biochemical test to determine a bacterial species ability to convert (breakdown) tryptophan into indole. Procedure: Test #1 1...

what is the purpose of the triple sugar iron test? - correct answer -the TSI test is designed to differentiate among the different groups or genera of the Enterobacteriaceae, which are all gram-negative bacilli capable of fermenting glucose with the production of acid, and to distinguish the Enterobacteriacase from other gram-negative intestinal bacilli. What is the enterobacteriacase? - correct answer -all gram negative bacilli capable of fermenting glucose with the production of acid. wh...

Lab 7 Notebook Title: SK07 Secondary Assays for Bacterial Characterization Objective: To profile a bacterial populations based on additional biochemical approaches to better determine growth/metabolic characteristics Procedure: Indole Test (Test #1) 1. Using a sterile look pick an isolated colony from BAP 2. Smear directly onto the reaction area of the slide 3. Examine test area for color change within 20 seconds Indole Test (Test #2) 1. Using a sterile loop pick an isolated colony fro...

Biochemically, the Enterobacteriaceae are gram negative rods that: - Answer Ferment glucose, reduce nitrate to nitrite, and are oxidase negative. THe ortho-nitrophenyl-Beta-galactopyranoside (ONPG) test is most useful when differentiating: - Answer Shigella spp. from some strains of Escherichia coli The Voges-Proskauer (VP) test detects which end product of glucose fermentation? - Answer Acetoin At which pH does the methyl red(MR) test become positive? - Answer pH of 4.5 ...