Bios 242 week 2 lab 2 - Study guides, Class notes & Summaries

Lab3:Microscopy-ObservationofCells LearningObjectives: • Usethecompoundlightmicroscopeforobservationofcells. • Identifydifferenttypesofbacteriabasedontheirshapes. • Identifyavarietyofeukaryoticcells. Introduction: Exercise2:ObservationofDifferentTypesofBacteriaMaterials: Compoundmicroscope,immersionoil,lenswipes,preparedslidesofvariousbacteria-Rods,cocciandspiral Method: 1. Obtainthepreservedslideofabacteria. 2. UseAppendixAandfollowtheinstructionsonhowtouseaMicroscope. 3. O...

BIOS 242 Week 6 Lab 2; Bacteria of Respiratory System

Lab2:TechniquesforIsolationofPureCultures LearningObjectives: • Isolatepureculturesfromamixedpopulationofmicroorganismsusingaseptictechnique. • Learnthestreakplatemethod Introduction: Inthislaboratoryexercise,coloniesfrommixturesofEscherichiacoliandSerratiamarcescenswillbeisolatedusingthefour-waystreakmethodassummarizedbelow(Figure1). We will need this image from Materials: A24hournutrientbrothmixtureofSerratiamarsecens(S.marsecens)andEscherichiacoli(E.coli),twonutrientagarpl...

Lab3:Microscopy-ObservationofCells LearningObjectives: • Usethecompoundlightmicroscopeforobservationofcells. • Identifydifferenttypesofbacteriabasedontheirshapes. • Identifyavarietyofeukaryoticcells. Introduction: Exercise2:ObservationofDifferentTypesofBacteriaMaterials: Compoundmicroscope,immersionoil,lenswipes,preparedslidesofvariousbacteria-Rods,cocciandspiral Method: 1. Obtainthepreservedslideofabacteria. 2. UseAppendixAandfollowtheinstructionsonhowtouseaMicroscope. 3. O...

BIOS 242 Week 7 Lab 2; Unknown Bacteria

Lab2:TechniquesforIsolationofPureCultures LearningObjectives: • Isolatepureculturesfromamixedpopulationofmicroorganismsusingaseptictechnique. • Learnthestreakplatemethod Introduction: Inthislaboratoryexercise,coloniesfrommixturesofEscherichiacoliandSerratiamarcescenswillbeisolatedusingthefour-waystreakmethodassummarizedbelow(Figure1). We will need this image from Materials: A24hournutrientbrothmixtureofSerratiamarsecens(S.marsecens)andEscherichiacoli(E.coli),twonutrientagarpl...

BIOS 242 Week 3 Lab 2; Carbohydrate Fermentation

Lab12:BacteriaoftheRespiratorySystem Introduction: Therespiratorysystemisdividedintotwoparts:theupperandlowerrespiratorysystems.Thelowerrespiratorysystemisanaxenicenvironmentbecauseof the cilia that lines the respiratory tract, alveolar macrophages and IgA antibodies found in the mucus. The upper part harbors a variety of microbiotasuch as Gram-negative bacteria Hemophilus, Gram-positive bacteria such as Staphylococcus aureus and diphtheroids that can cause opportunisticinfectionsleading toinf...

Lab10:HowGermsSpread Learningobjectives: • Tolearnhowgermsspread • Tobetterunderstandthespreadofgerms,byhealthcareprofessionals,frompatienttopatient • Tobetterunderstandtheimportanceofgoodhandwashing. Germsspreadinmanydifferentways.Directcontact,indirectcontact,aerosoltransmission,sexuallytransmittedorthroughavector.Inthislab,wewillobservehowgermsspread. Materials: Glowinthedarkgel,Blacklight Method: 1. Wearpersonalprotectiveequipmentincludinglabcoat,safetyglassesandgloves...

Lab8:FomiteTransmission LearningObjectives: • Definefomiteandhowbacteriaaretransferredviafomites. • Explaintheroleoffomitetransmissioninthehospital. • Definenosocomialinfections,HAIs(hospitalacquiredinfections),andIatrogenicinfections. Introduction: Materials:Agarplate,Marker,ruler,sterileswabs Method: 1. Obtainonenutrientagaranddividethebackoftheplateinto8sectionsusingrulerandmarker.Labeleachsection 1-8. 2. Usinganewsterileswab,swab8differentinanimateobjectsfoundinthelabareaa...