Polymerase - Study guides, Class notes & Summaries

GRAND CANYON UNIVERSITY NUR 631 ACCURATE 2023/2024 FALL SESSION FINAL EXAM.COMPLETE TEST WITH 100% CORRECT QUESTIONS AND ANSWERS GRADED A+ 1. A patient presents to the clinic with suspected carbon monoxide poisoning, O2 saturation is 99%. The NP understands that: a. The patient does not have carbon monoxide poisoning b. Carbon monoxide has already been flushed out of the body c. The patient needs supplemental oxygen d. The patient should be prepared for hyperbaric oxygen therapy 2. H...

We are going to use the blood sample you collected at the crime scene. What needs to happen before the blood sample can be used for PCR? a) DNA has to be isolated from the cells b) No action is needed c) You should shake the tube d) The blood has to be frozen. - Answer a) DNA has to be isolated from the cells What do you need to do each time before using pipette to collect liquid? a) Put on a used pipette tip b) Polish the pipette c) Shake the pipette d) Put on a new, sterile pipette tip -...

What needs to happen before the blood sample can be used for PCR? - Answer DNA has to be isolated from the cells What is the function of primers in a PCR reaction? - Answer Bind specific sites on the DNA For which enzymes are nucleotides the substrate? - Answer DNA polymerase What is the template of the PCR? - Answer DNA What does DNA polymerase do? - Answer synthesizes new DNA strands Why is it important to change the pipette tip? - Answer To avoid cross contami...

DNA Polymerase Study Guide Test. DNA Polymerase - CORRECT ANSWER Enzyme that assembles nucleotides into a new DNA strand DNA Polymerase I - CORRECT ANSWER DNA repair and some replication. Highest concentration DNA Polymerase II - CORRECT ANSWER DNA repair. Middle concentration DNA Polymerase - CORRECT ANSWER Have 3'-5' exonuclease; proofreading ability - keeps mutation rate below a certain level. Can only polymerize in a 5'-3' direction. Can only add nucleotides to an existing ...

What modification neutralizes the charges on histones and looses up the interactions between histones and DNA? - Answer- ACETYLATION Two types of post-transcriptional modifications that take place in the mRNA of eukaryotes - Answer- the addition of a CAP at the 5' end of the transcript and the addition of a POLY A sequence at the 3' end of the message Nucleic acid blotting is widely used in recombinant DNA technology. In a Southern blot one generally - Answer- Hybridizes filter-bound DNA...

uestion Number Answer Mark 1(a) The only correct answer is C a place where an organism lives A is incorrect because a group of organisms of different species living in the same area is a community B is incorrect because a group of organisms of the same species living in the same area is a population D is incorrect because the role of an organism is its niche (1) Question Number Answer Additional guidance Mark 1(b)(i) A description that makes reference to three of the following • ...

What needs to be done to the sample of blood before PCR? - Answer DNA must be isolated from the cells. How did you collect quids in the lab? - Answer A pipette What do you need to do each time before using a pipette to collect liquid? - Answer Put on a new, sterile pipette tip. What is the function of primers in a PCR reaction? - Answer Bind specific sites on DNA Great that you throw away the used pipette tip, why is it important to change the pipette tip? - Answer to ...

MB (ASCP) 2024 - Translocations, Diseases, Pharma, Polymerases, & more Chronic Myeloid Leukemia (CML) - Answer ️️ -t(9;22) BCR-ABL translocation Ewing sarcoma - Answer ️️ -t(11;22) EWS-FLI1 Acute Lymphocytic Leukemia (ALL) - Answer ️️ -t(9;22) BCR-ABL p190 protein Follicular Lymphoma - Answer ️️ -t(14;18) BCL2 translocation Mantle Cell Lymphoma - Answer ️️ -t(11;14) bcl-1 translocation CCND1-IgH Burkitt Lymphoma - Answer ️️ -t(8;14) MYC gene translocation R...

Fundamentals of Molecular Virology 2nd Edition by Acheson TEST BANK Package Title: Testbank Course Title: Acheson 2nd edition Chapter Number: 01 Question type: Multiple Choice 1) Which of the following is an enzyme that most RNA viruses encode in their genome? a) DNA-dependent-RNA-polymerase b) RNA-dependent-RNA-polymerase c) DNA-dependent-DNA-polymerase d) DNA-dependent-RNA-polymerase e) RNA-dependent-protein-synthetase Answer: b 2) Which of the following terms describes the prote...

The Bradford assay only works at low protein concentrations (from 0.05 to 0.5 mg/mL protein) How can we reduce the concentration of protein in our sample? - Answer dilute the sample what is the volume range of the P1000 - Answer 200 uL - 1000 uL how would you set the volume to pipette 20 uL with the P20? - Answer "200" how much protein do you need to add to the 800 uL buffer in the first microcentrifuge tube to make a 1:5 dilution? - Answer 200 uL how much fluid from th...