The purpose of pcr - Study guides, Class notes & Summaries

BIO 116 Lab Final Exam Study Guide Accuracy (Lab 4) - Ans:-measuring and dispensing the correct volume Precision (Lab 4) - Ans:-getting the correct volume to the correct, exact location Transgenic Crop - Ans:-Genetic material is transferred between 2 different species to achieve a desired trait Gene of interest - Ans:-yields desired characteristic Promoter - Ans:-noncoding segment of DNA upstream of the gene of interest; tells RNA polymerase where to start transcription Genetic marker - ...

What is the difference between the coding and non-coding region? - Coding region: comprised of genes that encode proteins (instructions that determine traits) and makes up 1-2% of human DNA. • Noncoding region: makes up 98-99% of human DNA, contains STRs and is responsible for regulatory functions such as gene transcription. What are STRs? - Short-tandem repeats (STRs) are unique repeating patterns of the same nucleotide sequence. They can be used to differentiate one person from another....

NYS EMT-B State Exam Written QUESTIONS AND CORRECT DETAILED ANSWERS WITH RATIONALES VERIFIED ANSWERS ALREADY GRADED A+ An EMS Provider responds to a call and finds an 8- yr old female with no pulse or respirations. The patient has a history of terminal brain cancer. The family states the last time she as seen alive was 5 mins prior to EMS being called. They also state patient has a DNR oder. In NYS, EMS personnel may accept which of the following DNR instruments as valid? Verbal reque...

how much BSA is needed to prepare 50 mL of 5 mg/ml of BSA (w/v)? - Answer-(50 mL)(5 mg,mL) = 250 mg a technician is assigned the task of inserting a gene, LanA, into a plasmid to ultimately be expressed as a therapeutic protein in E coli. label the following steps (1-4) in the correct order the technician must perform them to accomplish her task. - Answer-1) design primers to amplify the lanA sequence using polymerase chain reaction (PCR) and perform the PCR reaction 2) digest and provide ...

BIO 111 Lab Final Exam TAMU Questions And Answers 100% Solved | Graded A+ coding region - comprised of genes that encode proteins (instructions that determine traits) and makes up 1-2% of human DNA. non-coding gene - makes up 98-99% of human DNA, contains STRs and is responsible for regulatory functions such as gene transcription short tandem repeats (STRs) - unique repeating patterns of the same nucleotide sequence. They can be used to differentiate one person from another. DNA Profil...

The success of a PCR reaction is mostly dependent on what? Answer> well designed primers Primer design can also be applied to what? Answer> - probes in Southern and Northern blots - DNA sequencing - microarray design What else can affect the success of a PCR reaction? Answer> - Quality of the template DNA - cycling conditions - Specificity and stability of the PCR primers What are key criteria in primer design? Answer> - Primer length - Percent GC content - Melting temper...

BIO 111 Lab Final Exam TAMU Questions and Answers 100% Solved coding region - comprised of genes that encode proteins (instructions that determine traits) and makes up 1-2% of human DNA. non-coding gene - makes up 98-99% of human DNA, contains STRs and is responsible for regulatory functions such as gene transcription short tandem repeats (STRs) - unique repeating patterns of the same nucleotide sequence. They can be used to differentiate one person from another. DNA Profile - spe...

Coding regions - sequences of DNA that serve as instructions for making proteins (1-2% of human DNA) Non-coding regions - Human DNA that contains STRS and are responsible for regulatory functions ( DNA transcription 98-99% ) What are STRs? - short tandem repeats; unique repetition of nucleotide patterns. Differentiate one person from another. What is a DNA profile? - a specific pattern of DNA attributes that is obtained in the lab and can be used to identify an individual; is the process ...

BACE EXAM STUDY GUIDE QUESTIONS AND ANSWERS FULLY SOLVED What are the Common Career Technical Core - Career Ready Practices? 1. Act as a responsible and contributing citizen and employee. 2. Apply appropriate academic and technical skills. 3. Attend to personal health and financial well-being. 4. Communicate clearly, effectively and with reason. 5. Consider the environmental, social and economic impacts of decisions. 6. Demonstrate creativity and innovation. 7. Employ valid and reliable ...

FISDAP MEDICAL OPERATIONS PRACTICE TESTDuring the triage process, which of the following injuries or conditions would classify a patient as a high priority? A) Pulselessness and apnea B) Unilateral femur fracture and tachycardia C) Partial-thickness burns with no respiratory difficulty D) A large avulsion to the arm and an altered mental status - correct answer D During triage, patients with an altered mental status, who are in shock, or who have problems with their airway, breathing,...