Tsi agar slant - Study guides, Class notes & Summaries
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TSI Reactions Questions With Complete Solutions
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What sugars does triple sugar iron agar contain? correct answer: 1% Lactose 
1% Sucrose 
0.1% Glucose (Dextrose) 
 
What is TSI used for? correct answer: To differentiate enterics based on the ability to reduce sulfur and ferment carbohydrates 
 
What is fermented with a red (alkaline) slant and a yellow (acid) deep? (K/A) correct answer: Only glucose 
 
What is fermented with a yellow (acid) slant and a yellow (acid) deep? correct answer: Glucose and Lactose and/or Sucrose 
 
What is fermen...
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TSI Agar Exam 49 Questions with Verified Answers,100% CORRECT
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TSI Agar Exam 49 Questions with Verified Answers 
 
What is the agar used in the Triple Sugar Iron Agar - CORRECT ANSWER TSI (triple sugar agar medium) slant medium 
 
What does the TSI agar diffrentiate - CORRECT ANSWER it differentiates bacteria based on sulfur reduction and glucose, lactose, and sucrose fermentation 
 
What is the substrate used in the TSI experiment? - CORRECT ANSWER Sodium thiosulfate 
 
What is the enzyme used in the TSI experiment - CORRECT ANSWER Thiosulfate reductase 
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Triple Sugar Iron Test Exam 38 Questions with Verified Answers,100% CORRECT
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Triple Sugar Iron Test Exam 38 Questions with Verified Answers 
 
For what bacteria would you use the TSI test? - CORRECT ANSWER Gram negative bacilli such as enterobacteriaceae. 
 
How would you identify an acidic slant? - CORRECT ANSWER If the slant goes from red to yellow on the phenol red. 
 
Why is there more lactose and sucrose in TSI agar than glucose? - CORRECT ANSWER In order to distinguish bacteria that can only ferment glucose from bacteria that ferment lactose and sucrose. It will al...
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Triple Sugar Iron Agar (TSI) Questions and Answers 100% correct
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Triple Sugar Iron Agar (TSI) Questions and Answers 100% correct 
What is the TSI test used to determine? 
It is used to determine fermentation and sulfur reduction in Gram negative enteric bacteria 
 
 
 
What are the 3 sugars used in the TSIA test? 
Sucrose (present in high concentrations) 
 
Lactose (present in high concentrations) 
 
Glucose (present in low concentrations) 
 
 
 
K/A H2S+ 
 
 
 
K/K H2S- 
 
 
 
K/A H2S- 
 
 
 
A/A H2S- 
 
 
 
Control 
 
 
 
Sucrose 
Disaccharide 
 
 
 
Lactos...
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Triple Sugar-Iron Agar Test Questions with Verified Answers,100% CORRECT
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Triple Sugar-Iron Agar Test Questions with Verified Answers 
 
Gram- - CORRECT ANSWER Is TSI gram+ or gram- 
 
used to differentiate members of Enterobacteriacea and to distinguish them from other gram- rods - CORRECT ANSWER What is the application of TSI? 
 
1 glucose, lactose & sucrose 2 animal protein 3 ferrous sulfate 4 sodium thiosulfate 5 phenol red - CORRECT ANSWER What is the media in TSI (triple sugar iron) 
 
carbohydrate for fermentation - CORRECT ANSWER What is the purpose of glucose...
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Portage Learning BIOD 171 Lab Exams 1-8 Lecture Exams, 100% Accurate Answers provided.
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Portage Learning BIOD 171 Lab Exams 1-8 Lecture Exams, 100% Accurate Answers provided. 
Portage Learning BIOD 171 Lab Exams 1-8 Lecture Exams, 100% Accurate Answers provided. 
 
1. What three elements are used in an autoclave to sterilize equipment? 
heat, pressure, and steam 
2. What is the minimum temperature an autoclave must be set at to achieve sterile condition? 
125°C 
3. If you are working in a lab in which an autoclave is not available, and you are pressed for time, which would you c...
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PORTAGE LEARNING BIOD 171 LAB EXAM 1-9
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PORTAGE LEARNING BIOD 171 LAB EXAM 1-9 
 
LAB 1 
 
Answer the following questions 
 
1.	What three elements are used in an autoclave to sterilize equipment? 
 
heat, pressure, and steam 
 
2.	What is the minimum temperature an autoclave must be set at to achieve sterile condition? 
 
125°C 
 
3.	If you are working in a lab in which an autoclave is not available, and you are pressed for time, which would you chose to best sterilize your equipment? Hot steam or hot air? Explain why you chose your...
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TSI (Triple Sugar Iron) Test 32 Questions with Verified Answers,100% CORRECT
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TSI (Triple Sugar Iron) Test 32 Questions with Verified Answers 
 
List the tests that are involved with TSI. - CORRECT ANSWER H2S Gas and Carbohydrate Fermentation 
 
What is the media for H2S Gas? - CORRECT ANSWER TSI agar 
 
What method do you use to place content on the media? - CORRECT ANSWER stab and drag motion 
 
what is the substrate for H2S gas? - CORRECT ANSWER cystein 
 
What is the product for H2S gas? - CORRECT ANSWER H2S 
 
What is the reagent for H2S gas? - CORRECT ANSWER iron in...
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TSI Tests 37 Questions with Verified Answers,100% CORRECT
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TSI Tests 37 Questions with Verified Answers 
 
Red slant/ yellow butt - CORRECT ANSWER Glucose only. Protein used in slant 
 
Yellow slant/yellow butt - CORRECT ANSWER -Glucose + 
-Lactose and sucrose + 
 
Red slant/ red butt - CORRECT ANSWER -no fermentation 
Protein catabolized throughout 
 
Red slant only - CORRECT ANSWER Aerobic digestion of protein only 
 
No change in slant or butt - CORRECT ANSWER Slow or no growth 
 
Yellow slant/red butt - CORRECT ANSWER Not possible 
 
Black precipita...
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BIOD 171 Lab Exam 7 with complete solutions
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What color(s) was the TSI agar slant containing Salmonella? 
red slant, yellow bottom and no gas production. 
Yellow and Black. The agar slant was yellow at the bottom while black on the sloped gradient 
portion of the agar.
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