Bsc4422 - Study guides, Class notes & Summaries

Western blotting a. Proteins from SDS-PAGE are transferred to a nitrocellulose membrane using electrical current a. membrane is blocked so antibody will not bind nonspecifically b. Primary antibody is added and incubated with the membrane c. To detect antibodies that have bound to protein a secondary anti-immunoglobulin antibody coupled to a reporter a. Excess secondary antibody is washed off the membrane b. Substrate is added that precipitates upon reaction c. Reveals visible band where...

Creating DNA Libraries -Collections of cloned DNA fragments from a particular organism contained in plasmid vectors within host bacteria -Screen library to pick out different genes of interest Genomic DNA Libraries -Used to locate fragment with DNA of interest -Chromosomal DNA from tissue of interest is isolated and digested using a restriction enzyme (sticky/blunt ends) -Vector also digested -DNA ligase used to ligate chromosomal DNA fragment inside vector DNA (plasmids) -Recombinant ...

500 powerpoint slides compiled into 23 pages.

This study guide includes all class notes needed to study for Exam 1 in BSC 4422 (Biotechnology) with Professor Paul R. Sharp at Florida International University.

BSC4422 Exam 3 Review Notes

Modern examples of biotechnology

The notes contains the seven types of biotechnology and some examples of historic ways that biotechnology was used.

BSC 4422 Exam 3 Review Notes

BSC 4422 Exam 4 Review

BSC 4422 Exam 1 Review Notes