Northern hybridization - Study guides, Class notes & Summaries

MCB 2050 Midterm W21 – Dr. Yankulov via Respondus Lockdown Browser and Webcam PART A – Questions from tutorial Question 1. You are studying the genes involved in embryonal development. Which of the following techniques could be used to visualize the locations with high gene expression of a particular gene in a whole mounted embryo? a) Western blotting b) Northern blotting c) Southern blotting d) Immunoprecipitation e) RNA in situ hybridization Question 2. Why is Taq DNA polymerase ...

E. coli RNA Polymerase II - Answer DNA template, product: mRNA RNA Polymerase I - Answer DNA template Location: Nucleous Products: 18s, 5.8s, 28s rRNA RNA Polymerase II - Answer DNA template Location: Nucleus Products: mRNA, snRNA RNA Polymerase III - Answer DNA template Location: Nucleus Product: tRNA, 5s rRNA, snRNA Mitochondrial RNA Polymerase - Answer DNA template, product: mRNA Mammalian DNA polymerase α - Answer DNA template, product: primers HCV RNA Polymerase - A...

MICR 351 TESTED QUESTIONS WITH ALL ANSWERS AND ALL PRINTED PAGES Northern Hybridization? - Answer-mRNA-cDNA hybridization to find gene of interest using DNA probe to identify which bond holds gene of interest Western Hybridization? - Answer-Antibody-protein hybridization, where antibodies bind to a specific protein bond that contains target protein that produces antibody - used in vaccines and other tests, such as pregnancy tests Functional genomics - Answer-Microarrays and RNA-sequence...

The pathophysiology of Glazmann thrombasthenia is: A. Deficiency of dense granules B. Deficiency of glycoprotein Ib/IX receptor C. Deficiency of glycoprotein IIb/IIIa receptor D. deficiency of microtubules - C. Deficiency of glycoprotein IIb/IIIa receptor Chagas disease is caused by: A. Trypanosoma cruzi B. Trypanosoma palladium C. Trypanosoma brucei D. None of the above - A. Trypanosoma cruzi What would a patient with a microcyctic/hypochromic anemia most likely have noted on their CB...

BMSC 210 Midterm 2 | 100% Correct Answers | Verified | Latest 2024 Version What is genetic engineering? - using in vitro techniques to alter genetic material in the lab What is recombinant DNA technology? - the artificial recombination of DNA from two organisms What are some basic techniques of genetic engineering? - DNA amplification electrophoesis nucleic acid hybridization molecular cloning expressing foreign genes targeted mutagenesis What is heterologous expression? - expr...

A micoarray is testing for: - Answer a lot of genetic characteristics from one sample Southern Hybridization - Answer extract DNA probe w/ DNA Northern Hybridization - Answer extract RNA probe w/ DNA Western Hybridization - Answer extract proteins probe with antibodies What is the first method that became standard in DNA sequencing? - Answer Sanger method What type of PCR does Sanger Method use? - Answer cycle sequencing PCR Cycle Sequencing PCR requirements -...

Tools used in biotechnology and genetic engineering - Answer-In Silico analysis, DNA microarray analysis, gel electrophoresis, PCR, and RFLP analysis. PCR - Answer-*Polymerase Chain Reaction* technique for quickly and easily making many copies of even a very small amount of DNA Reverse Transcriptase - Answer-enzyme that turns single-stranded RNA into double-stranded DNA Complementary DNA (cDNA) - Answer-DNA produced from an RNA template using reverse transcriptase. often used to clone ...

Chapter 3 Recombinant DNA Technology and Genomics Exam Questions With Verified Answers. Stone-age genomics - answera number of laboratories around the world are involved in analyzing "ancient" DNA. These studies are generating fascinating data from miniscule amounts of ancient DNA from bone and other tissues and fossil samples that are tens of thousands of years old. bacteriophage - answera virus that is parasitic in bacteria origins of replication - answerspecific locations in a DNA m...

GENE 4200 practice exam 1|26 Questions with Verified Answers Transcription factors help regulate which cell types and tissues will express a given gene (T/F) - CORRECT ANSWER When comparing RNA levels for gene X between two samples by qPCR, the sample with thehighest DCT value would have the least gene X RNA (T/F) - CORRECT ANSWER Exons are the most abundant DNA sequences in the human genome (T/F) - CORRECT ANSWER false Most transposons in the human genome are inactive. (T/F) - COR...

Central Dogma DNA → RNA → Protein Purines - which bases? - how many rings? Brainpower Read More 0:14 / 0:15 Pyrimidines - which bases? - how many rings? Remember: "Pyrimidine" has a "y" in it; so does "cytosine" and "thymine." Phosphodiester Bond Joins one nucleotide to the next; between the 3rd C of one ribose and the 5th C of the other to create the sugar-phosphate backbone Directionality of DNA (convention) 5' → 3' (but strands are ant...