Technical Skills and
Applications: BACE Version B
questions and answers
confocal microscope - answer uses a laser light to scan samples that
have been dyed
inverted microscope - answer light source is located above the
specimen
bright field microscope - answer objectives are located above the
stage
electron microscope - answer uses electrons to scan samples in
near-vacuum conditions
what makes a good primer? - answer
what would increase the likelihood for good separation of adjacent
bands on an agarose gel? - answer using a higher percentage of
agarose in the gel
what is genetic engineering? - answer lab-based technology that
allows the manipulation of the genetic makeup of an organism
what is the best experimental design for optimizing the primer-
annealing temperature? - answer multiple reactions run with primer,
DNA,dNPT's, and buffer concentration kept constant, changing only
the annealing temperature in a range of 50-70 degrees Celsius
Applications: BACE Version B
questions and answers
confocal microscope - answer uses a laser light to scan samples that
have been dyed
inverted microscope - answer light source is located above the
specimen
bright field microscope - answer objectives are located above the
stage
electron microscope - answer uses electrons to scan samples in
near-vacuum conditions
what makes a good primer? - answer
what would increase the likelihood for good separation of adjacent
bands on an agarose gel? - answer using a higher percentage of
agarose in the gel
what is genetic engineering? - answer lab-based technology that
allows the manipulation of the genetic makeup of an organism
what is the best experimental design for optimizing the primer-
annealing temperature? - answer multiple reactions run with primer,
DNA,dNPT's, and buffer concentration kept constant, changing only
the annealing temperature in a range of 50-70 degrees Celsius
