Summary

Summary Chapter 3.3

5 views 0 purchase

Course
Biochemistry I (BIOL2080U)

Institution
University Of Ontario Institute Of Technology (UOIT )

Summary study book Lehninger Principles of Biochemistry of Nelson David L., Albert L. Lehninger, David L. Nelson, Michael M. Cox, University Michael M Cox (3.3) - ISBN: 9780716743392 (Chapter 3.3)

[Show more]

Preview 1 out of 4 pages

View example

Summarized whole book? No
Which chapters are summarized? 3.3
Uploaded on May 28, 2021
Number of pages 4
Written in 2020/2021
Type Summary

CA$10.88

Added

Add to cart Add to wishlist

100% satisfaction guarantee
Immediately available after payment
Both online and in PDF
No strings attached

Chapter 3.3 Part 2 – Lecture #17

3-5 A Purification Table for a Hypothetical Enzyme

FIGURE 3-18 Electrophoresis. (a) Different examples are stacked in wells or
discouragements at the highest point of the SDS polyacrylamide gel. The proteins
move into the gel when an electric field is applied. The gel limits convection flows
brought about by little temperature angles, just as protein developments other than
those instigated by the electric field. (b) Proteins can be envisioned after
electrophoresis by treating the gel with a stain, for example, Coomassie blue, which
ties to the proteins however not to the actual gel.

Each band on the gel addresses an alternate protein (or protein subunit); more
modest proteins travel through the gel more quickly than bigger proteins and
accordingly are discovered closer the lower part of the gel. This gel delineates
decontamination of the RecA protein of Escherichia coli (portrayed in Chapter 25).

The quality for the RecA protein was cloned (Chapter 9) with the goal that its
demeanor (amalgamation of the protein) could be controlled. The primary path
shows a bunch of standard proteins (of referred to Mr), filling in as sub-atomic
weight markers. The following two paths show proteins from E. coli cells when
combination of RecA protein was actuated. The fourth path shows the proteins in an
unrefined cell separate. Resulting paths (left to right) show the proteins present
after progressive refinement steps. The cleaned protein is a solitary polypeptide
chain (Mr ~38,000), as found in the furthest right path.

Electrophoresis of proteins is by and large completed in gels comprised of the cross-
connected polymer polyacrylamide (Fig. 3-18). The polyacrylamide gel goes about
as an atomic sifter, easing back the movement of proteins around in relation to
their charge-to-mass proportion. Movement may likewise be influenced by protein
shape. In electrophoresis, the power moving the macromolecule is the electrical
potential, E. The electrophoretic versatility, μ, of an atom is the proportion of its
speed, V, to the electrical potential. Electrophoretic portability is likewise equivalent
to the net charge, Z, of the particle isolated by the frictional coefficient, f, which
reflects to some extent a protein's shape. In this manner:

V Z
μ= =
E f

The relocation of a protein in a gel during electrophoresis is along these lines a
component of its size and its shape. An electrophoretic strategy generally utilized
for assessment of immaculateness and sub-atomic weight utilizes the cleanser
sodium dodecyl sulfate (SDS) ("dodecyl" signifying a 12-carbon chain).

A protein will tie about 1.4 occasions its weight of SDS, almost one atom of SDS for
every amino corrosive buildup. The bound SDS contributes a huge net negative
charge, delivering the inborn charge of the protein unimportant and presenting on
every protein a comparative charge-to-mass proportion. Furthermore, SDS

The benefits of buying summaries with Stuvia:

Guaranteed quality through customer reviews

Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.

Quick and easy check-out

You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.

Focus on what matters

Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!

Frequently asked questions

What do I get when I buy this document?

You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.

Satisfaction guarantee: how does it work?

Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.

Who am I buying these notes from?

Stuvia is a marketplace, so you are not buying this document from us, but from seller kuganesh02. Stuvia facilitates payment to the seller.

Will I be stuck with a subscription?

No, you only buy these notes for CA$10.88. You're not tied to anything after your purchase.

Can Stuvia be trusted?

4.6 stars on Google & Trustpilot (+1000 reviews)

77254 documents were sold in the last 30 days

Founded in 2010, the go-to place to buy study notes for 14 years now

Start selling

Summary

Summary Chapter 3.3

Document information

Subjects

Connected book

More summaries for

Written for

Seller

Content preview