Other

Pharmacology practical report

35 views 3 purchases

Course
Pharmacology practical (WBFA03605)

Institution
Rijksuniversiteit Groningen (RuG)

This report was graded with a 7.5 and will elaborate on the findings from the lab, but could mostly suffice as a setup for your own report in terms of content organization, results processing and discussion, and conclusion writing.

[Show more]

Preview 4 out of 37 pages

View example

Uploaded on January 10, 2023
Number of pages 37
Written in 2022/2023
Type Other
Person Unknown

receptoren
lab
organ bath
medicijnen
report
verslag
experimental results
compound a
compound b
compound c
contraction
relaxation
deduction and finding out about medicines

Institution
Rijksuniversiteit Groningen (RuG)
Education
Farmacie
Course
Pharmacology practical (WBFA03605)

tymozwakenberg

Member since 1 year 22 documents sold

CA$23.59

Added

Add to cart

Add to wishlist

100% satisfaction guarantee
Immediately available after payment
Both online and in PDF
No strings attached

In vitro identification of unknown compounds A, B and C in a
pharmacology practical.

Tymo Zwakenberg S4326253

Group 16

Date of experiments: 26-10-2022, 3-11-2022 and 22-11-2022

Date of submission:

Dr. H.A. Baarsma

Ing. A.B. Zuidhof

1

,Table of contents
- Introduction and theory
o Objective
o Theoretical principles
o Ileum
o Aorta
o Atrium
- Materials and methods
o Organ baths
o Execution and calculation examples
- Results
o Ileum
▪ Compound A
▪ Metacholine response curve
▪ Compound A + metacholine
▪ Compound B
▪ Compound C
o Aorta
▪ Compound A, B & C
▪ Phenylephrine loading dose curve
▪ Compound A + phenylephrine
▪ Histamine loading dose curve
▪ Compound B + histamine
▪ Phenylephrine loading dose curve
▪ Compound C + phenylephrine
▪ Histamine loading dose curve
▪ Compound C + histamine
o Atrium
▪ Histamine loading dose curve
▪ Compound B + histamine
▪ Compound C
▪ Compound C + mepyramine
▪ Compound C + ketanserin
- Discussion and conclusion
o Experimental day 1
▪ Inclusion/exclusion of possibilities
o Experimental day 2
▪ Inclusion/exclusion of possibilities
o Experimental day 3
▪ Inclusion/exclusion of possibilities
o In vivo
o Compound A
o Compound B
o Compound C
- References

2

,- Appendices
o Appendix A: Ileum
▪ Histamine dose response curves
• Appendix figure 1
• Appendix figure 2
• Appendix figure 3
• Appendix figure 4
▪ Appendix calculations
▪ Table of raw data
o Appendix B: Aorta
▪ Histamine dose response curves
• Appendix figure 5
• Appendix figure 6
• Appendix figure 7
• Appendix figure 8
▪ Appendix calculations
▪ Table of raw data
o Appendix C: Atrium
▪ Histamine dose response curves
• Appendix figure 9
▪ Appendix calculations
▪ Table of raw data

3

, Introduction and theory
Objective
The execution of the pharmacology practical designed like this should provide in the development of
pharmacological principles existing of independently setting up experimental ideas, researching on
animal organ material in organ baths, organizing group coöperation, working with agonistic and
antagonistic compounds and ultimately indentifying unknown compounds. The execution of the
practical should setup experimental executioners for further pharmacological experiments and
provide the basis which is necessary in that regard. Each experimental day provides the chance to
get closer to the identity of the unknown compounds. However, although the identification of the
compounds is a central part of the practical, the reasoning and thought processes that will be the
backbone of the results and discussion are at least – if not even more important than the final
compound identification itself. Since the practical should provide in the knownledge of
pharmacological aspects and not as much about the characteristics of the unknown compounds, the
reasoning and thought processes that will be discussed should be considered heavily in order to get
a better grip on this pharmacology practical and possible upcoming pharmacological practicals.

Theoretical principles
Before the practicals can be attended, knowlegde about the known compounds, experimental setup
of the organ baths, possible targetable receptors, characteristics of the particular organ and the goal
of the day should be sufficient. Preparation and the knowledge about the stated components are
necessary in order to succeed in the execution of the practical. This and the fact that animal organ
material is used should stimulate personal responsibility to be prepared and work accordingly. The
information that was used throughout the practicals will be discussed along the results and
discussion section in order to provide the explanations.

All the substances that are provided and used throughout the practical, whether it is a known
compound or an unknown compound resemble an agonistic or antagonistic influence on the organ
samples. An agonistic influence would result in the contraction of the muscle that is present in that
specific organ. On the other hand should the antagonistic influence not show contracting
characteristics but relaxational characteristics, this is mostly observable after a contraction has
occured. However, the antagonistic influence can also be indicated through the occurance of a right
shift in agonistic concentration in order to exert contraction. To clarify this the stated effects of an
agonist and antagonist are represented in figure 1 & 2.

Figure 1: Characteristic concentration-effect curve after the addition of increasing agonist
concentrations [4].

4

The benefits of buying summaries with Stuvia:

Guaranteed quality through customer reviews

Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.

Quick and easy check-out

You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.

Focus on what matters

Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!

Frequently asked questions

What do I get when I buy this document?

You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.

Satisfaction guarantee: how does it work?

Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.

Who am I buying these notes from?

Stuvia is a marketplace, so you are not buying this document from us, but from seller tymozwakenberg. Stuvia facilitates payment to the seller.

Will I be stuck with a subscription?

No, you only buy these notes for CA$23.59. You're not tied to anything after your purchase.

Can Stuvia be trusted?

4.6 stars on Google & Trustpilot (+1000 reviews)

81989 documents were sold in the last 30 days

Founded in 2010, the go-to place to buy study notes for 14 years now

Start selling

Other

Pharmacology practical report

Document information

Subjects

Written for

Seller

Reviews received

Content preview