BISC 440 cell and molec final questions answered 100% correct

BISC 440 cell and molec final questions answered 100% correct What would be the best positive control for the transformation? different plasmid with the same backbone What happens if we grow the bacteria in a medium containing chloramphenicol? cells with the plasmid will survive What is the purpose of the antibiotics in the agar plate? selection What is the green gene on our plasmid (amilCP)? reporter gene What would the sticker ends of your BioBrick part look like if you cut our plasmid with Xbal (T'CTAGA) and Psti (CTGCA'G)? CTAG and TGCA Which restriction enzyme produces the same overhand as Xbal? Spel Which of the following sticker ends would be fused to your BioBrick part by the ligase? 5' CTAGTAGC (BioBrick part 2) 3' ATCG (BioBrick part 2) (BioBrick part 1) GCTT 3' (BioBrick part 1) CGAAGATC 5' Which of the following restriction enzymes could be ligated together? Xbal and Spel Why do we heat the tube to 80 C? inactivate enzyme Now our parts are cut. How can we combine them together? use a ligase What is NOT an advantage of the 3A assembly? easy gel purification What is your observation when looking at the two images? increased number of stained blood vessels (brown) in the ductal carcinoma What does the signaling protein VEGF stimulate? mitosis Why is cancer growth dependent on an increased number of blood vessels? to supply the tumor with oxygen and nutrients How can we easily determine VEGF expression in a western blot experiment? by using a primary antibody targeting VEGF In addition to the anti0VEGF antibody, Marie has decided to also add a VEGFR primary antibody. At which position do you expect the band for VEGFR to appear? Remember that in SDS-PAGE proteins are separated according to their molecular weight. VEGFR runs at a higher molecular weight than WEGF Looking at the western blot result. What is your conclusion for the patient samples? VEGF and VEGFR are overexpressed As you can see, Marie also added an anti-beta-actin antibody. Why do you think this was important? to check if the same amount of sample was loaded on the gel In which other type of cancers do you expect VEGF signaling to play a role? in the progression of most solid tumors What property prevents the ligands of cell surface receptors from entering the cell? Remember that the ligands are usually secreted into the extracellular fluid or the blood-stream. hydrophobicity What does a target cell require to respond to an extracellular signal molecule? -access to the ligand -matching receptors -activation of effector proteins