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BIOL 10016 Experiment 9 Factors Affecting Enzyme Function (2023)(NEW)
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MOHAWK COLLEGE OF APPLIED ARTS AND TECHNOLOGYCHEMICAL ,ENVIRONMENTAL & BIOTECHNOLOGY DEPARTMENT
Lab Report
ROOM NO: FE E309
Class : BIOL 10016 Lab.
EXPERIMENT NO : 9
TITLE : Factors Affecting Enzyme Function
Submitted by :
Partners :
Instructor : Farag Soliman
Date lab performed : February 17, 2023
Date of submission : February 24, 2023
FENNELL CAMPUS
HAMILTON, ONTARIO
1
, Purpose:
The purpose of this experiment was to teach the student how enzymes react with
different pH values under the same conditions. This experiment explained how
starch reacts with enzyme Amylase to produce a Maltose sugar and how
differences in pH affects the starch hydrolysis. The experiment will also allow the
students to recognize the optimal temperature and pH of the enzyme and its
differences with other samples at the end of the experiment.
Procedure:
Biotechnology 1 Laboratory Manual BIOL 10016 Lab. Experiment 9 Factors
Affecting Enzyme Functions. Pages 2-3.
Materials
Tools
15 mL Tubes
Tube rack
Beakers
Ice Box
Crucible Tong
Graduated Cylinder
Reagents
Maltose Colour Reagent (DNS solution)
Concentrated Amylase stock solution
1% starch solution
Instrument
Novaspec III
Laboratory Hot Plate
Vortex
Micropipette
2
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