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BIOL 10016 Biotechnology - Experiment 6 Total Protein Assay (2023)(NEW)
BIOL 10016 Biotechnology - Experiment 6 Total Protein Assay
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MOHAWK COLLEGE OF APPLIED ARTS AND TECHNOLOGYCHEMICAL ,ENVIRONMENTAL & BIOTECHNOLOGY DEPARTMENT
Lab Report
ROOM NO: FE E309
Class : BIOL 10016 Lab.
EXPERIMENT NO : 6
TITLE : Total Protein Assay
Submitted by :
Partners :
Instructor : Farag Soliman
Date lab performed: March 31, 2023
Date of submission: April 7, 2023
FENNELL CAMPUS
HAMILTON, ONTARIO
1
, Purpose
A total protein assay is a biochemical method used to determine the total amount
of protein present in each sample. The purpose of this assay is to provide an
accurate measurement of the protein content of the sample, which can be useful
for a variety of applications. For example, total protein assays are commonly used
in medical diagnosis to assess a patient's overall health, as high levels of protein in
the urine or blood can be indicative of kidney disease or other medical conditions.
In addition, total protein assays can be used in protein research to quantify the
amount of protein present in a sample, which is important for a variety of
downstream applications. Total protein assays can be performed using a variety of
methods, including colorimetric assays, which rely on the reaction between a
protein and a specific dye to produce a color change that can be measured using a
spectrophotometer. Another common method for performing a total protein assay
is the biuret method, which involves the reaction between a protein and a copper
ion. The resulting complex absorbs light at a specific wavelength, allowing the
amount of protein in the sample to be measured. Overall, total protein assays are
a powerful tool for quantifying the protein content of a sample and are essential in
many areas of research and diagnosis.
Procedures
Mohawk College. 2010. Biotechnology 1 Laboratory Manual BIOL 10016 Lab.
Experiment 6 Total Protein Assays.
Materials:
Tools
Beaker
Test Tubes
Micropipette
Graduated Cylinder
Plastic Cuvettes
Parafilm
Pasteur Pipette
Reagents
Bradford Reagent
50 mL Methanol
100 mL of 85% H3PO4
Norit 100 mg
Coomassie Blue G 100 mg
Instrument
HACH DR 6000 UV-visible Spectrophotometer
2
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