Case
ECOLI. ASSIGNMENT
- Course
- BI208
Case of 8 pages for the course BI208 at (ECOLI. ASSIGNMENT)
[Show more]
Seller
Follow
Content preview
TITLE - Escherichia coliSUBTITLE - Characteristics, Identification, Isolation, Significance and Biochemical test
profile for Escherichia coli
Gurleen Kaur
School of Engineering Technology and Applied Science
Centennial College
Microbiology Project 1 (BI-207) - 103
Rochelle Fleming
19 th February,2023
, Characteristics
1. Microscopic Morphology
● Escherichia coli (E. coli) are straight, gram-negative cylindrical rods.
● They are generally 1-1.5μm in diameter and 2.0-6.0μm in length and appear
alone or in pairs. Escherichia coli (E. coli) are from Enterobacteriaceae
family and are Non-motile or motile via peritrichous flagella (Brenner et
al.,2005).
2. Colonial morphology
● The phenotypes of strains grown on agar media are classified as smooth (S) or
rough (R) according to the amount of O antigen polysaccharide polymerized.
● While R forms typically grow as flat, dry, dull, wrinkled colonies (1- 5 mm
diameter) with a blurred edge on agar and agglutinate spontaneously in fluid
media, they have lost their polysaccharide side chains through mutation.
● S forms typically grow on nutrient agar as convex, glistening, moist, grey
colonies (2-3 mm diameter) with a defined edge or in fluid medium as turbid
growth (Brenner et al.,2005).
3. Atmospheric requirements & nutritional requirements
● Strains of E. coli survive and develop throughout the medium range (15–45C).
● E. coli may grow in the mid-range of pH, or between 5.0 and 9.0.
● There are anaerobic biotypes, although most organisms are aerobic and
facultatively anaerobic, that have both a respiratory and a fermentative form of
metabolism.
● oxygenase negative.
● Chemoorganotrophic (Brenner et al.,2005).
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller gurleenkaur. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for CA$12.98. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
75323 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now