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Molecular Genetics Exam Questions
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Which of the following correctly describes the shapes of the major and minor grooves in double-helical RNA? The major groove is narrow and deep - The major groove is narrow and deep and the minor groove is wide and shallow. The underlying principle of "classical" methods of DNA sequencing is ...

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  • August 2, 2023
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  • 2023/2024
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Molecular Genetics Exam Questions


Which of the following correctly describes the shapes of the major and minor grooves in double-helical
RNA?

The major groove is narrow and deep - The major groove is narrow and deep and the minor groove is
wide and shallow.



The underlying principle of "classical" methods of DNA sequencing is to separate, by size, nested sets of
DNA molecules. There are two main steps involved: (1) running a PCR-like sequencing reaction and then
(2) running the extension products through a gel matrix to separate them by size. The reagents used in
the sequencing reaction are similar to those used in PCR, yet there are some important differences.
What are the main reagents of a sequencing reaction? - dNTPs, ddNTPs, one primer, DNA polymerase



The "intergenic" parts of the human genome consist of which types of DNA sequences? - genome-wide
repeats, micro-satellite DNA, and microRNAs



Which of the following belong to the M phase of the cell cycle? - anaphase, metaphase, prophase



A single histone H1 binds to which of the following? - linker DNA on one side of a nucleosome and a
second site in the middle of the DNA wrapped around the nucleosome



Which of the following is/are possible "post-translational" modification that can occur on a histone
subunit? - methylation, phosphorylation, acetylation



which of the following description is/are not accurate? - chromosome often has a banding pattern that
happens naturally, the darker region of the chromosomal band is called the R band, the lighter band is
called the R band, the lighter region of the chromosomal band is often A-T rich and is the region packed
with genes, telomere is likely to be shortened after DNA replication. if the telomere is truncated, the cell
will undergo apoptosis



Pick the reasons why second-generation sequencing can sequence a whole genome faster than Sanger
sequencing - perform multiple DNA sequences read simultaneously, perform creation without added
ddNTPs, stitch together sequential DNA sequence through bioinformatics analysis

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