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General Biochemistry test questions

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  • BIOCHEMISTRY.
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  • BIOCHEMISTRY.

General Biochemistry test questions Convergent evolution would be expected to yield... Proteins of clearly different three dimensional structure and the same or similar function. An experimenter believes that an inhibitor of a Michaelis-Menten enzyme binds to ES complex but not to free enz...

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  • January 22, 2024
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  • 2023/2024
  • Exam (elaborations)
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  • BIOCHEMISTRY.
  • BIOCHEMISTRY.
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General Biochemistry test questions
Convergent evolution would be expected to yield... - ANSWER Proteins of clearly different three dimensional structure and the same or similar function.
An experimenter believes that an inhibitor of a Michaelis-Menten enzyme binds to ES complex but not to free enzyme. She would expect that. - ANSWER Lineweaver Burke plots of the data would have parallel lines
The half time of a first order reaction is the time required for - ANSWER Half the amount of reactants present to bensure consumed.
The observation that amino acid volumes calculated by the Voronoi method are essentially the same for buried residues in proteins and for crystalline amino acids implies that.. - ANSWER The protein interior is
closely packed with little empty space within the folded molecule
Serine is found at positions 77 and 79 of a normal, wild type protein. In a series of site specific mutagenesis experiments, all nineteen of the other amino acids are substituted for ser 79. The unfolding
temperature of all thirty-eight mutant proteins is compared with that of the wild type. At position 77 only alanine had no effect on the unfolding temperature. All other amino acids lowered the Tm. At position 79, however, not only alanine but also eleven other amino acids had no effect on the stability. Which of the following best explains these results? - ANSWER Residue 79 is on the protein surface and interacts with few other residues, while residue 77 is an interior residue and normally interacts with many other residues in the three dimensional structure
In am enzyme catalyzed reaction in the absence of any inhibitor, the substrate concentration is half the value of Km. The percent of the active sites in the reaction mixture which is ocupid by substrate at any given time is - ANSWER 25%
Rotations about the peptide bond C-N I'm proteins (w rotations) are severely limited because - ANSWER Pi electrons give the bond partial double bond character

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