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QBM UCF Final Exam

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QBM UCF Final Exam Assorted terms Molecular Biology The study, analysis, and manipulation of DNA and proteins. Recombinant DNA Technology The process of taking a gene from one source and joining it with another piece of DNA and inserting that DNA into bacteria for further study and manipula...

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  • May 9, 2024
  • 11
  • 2023/2024
  • Exam (elaborations)
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  • qbm ucf final exam assorted terms molecular biolog

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QBM UCF Final Exam Assorted terms
Molecular Biology
The study, analysis, and manipulation of DNA and proteins.


Recombinant DNA Technology
The process of taking a gene from one source and joining it with another piece of DNA and inserting
that DNA into bacteria for further study and manipulation


OSHA
Occupational Health and Safety Administration
State that employers must inform employees about any potential work place hazard.


MSDS
Sheet used to describe a chemical's physical and chemical properties and any potential hazards it may
present.


CDC
Center for Disease Control
Regulates any research that involves disease-causing agents


BSL 1
Environment presents limited hazards to any researchers/employees


BSL 2
Labs that present researchers with moderate risks. Any employees must be trained and certified to
work with equipment and any hazardous chemicals need to disposed of properly.
Most labs on UCF campus are BSL 2


BSL 3
Labs that work with pathogenic agents capable of causing disease. Require proper ventilation


BSL 4
Labs that work with dangerous pathogens that cause fatal diseases and for which there are no
vaccines.


PPE
Personal Protective Equipment
Gloves, Labcoat, Goggles
Latex gloves are best

Note:
Never recap needles


Graduated Cylinders
Large volumes of 25 mL to 2 L

, Pasteur Pipetts
Transfer pipette used when specific volumes are not required.


Micropipettes
Most accurate measuring device


Centrifuge
Separates solutions based on density. Require precise balancing.
g = Relative Centrifugal Force (universal)


Analytical Centifugation
Sedimentation characteristics of pure samples can be analyzed.
Used to determine purity,molecular weight, size and diffusion coefficients.
Ultracentrifuge


Large-capacity Centrifugation
Used to separate large volumes (up to 2 L) of bacteria.
capable of reaching 6500 g


High-speed Centrifugation
Used for volumes of about 50 mL.
Separate microorganisms and cellular debris


Fixed-angle Rotors
Perfect for spinning pellets


Swinging-bucket Rotors
Analytical
Used for cesium chloride gradients.

Used by Messelson-Stahl to analyze DNA replication (semi-conservative)


Clear bottles
Better visualization but are less resilient to cracking and chemicals


Spectrometry
The study of radiation interacting with matter


Spectrophotometers
Measure the intensity of the wave length of light as it passes through a sample solution.
Measures absorbance


Plate Readers
Advanced spectrophotometers that can measure the absorbency of hundreds of samples at once
Used in ELISAs, drug screenings, and enzyme assays.

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