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Microbiology Media Guide

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Course
Medical Laboratory Science

Institution
Medical Laboratory Science

**NOTE** This document is best viewed in landscape format, though the preview pages default to portrait. This list is limited to media you would use in a medical microbiology lab and some you would use in the food or bio-pharm industries. All information is displayed in an easy to read, color-...

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Uploaded on June 20, 2024
Number of pages 5
Written in 2023/2024
Type Other
Person Unknown

microbiology
media guide
reference guide
lactose fermenters
table
chart
microbiology media
medical lab
csmls
health sciences
biotechnology
food micro
selective media
differential media
mlt
mla
microor

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MEDIA TYPE SAMPLE NOTABLE
NAME WHAT IT DOES
INGREDIENTS TYPES ORGANISMS
Enriched and Almost ALL Streptococcus spp., Grows fastidious organisms
Differential: 5% samples Pseudomonas Produces hemolytic patterns which can
Sheep Sheep Blood get a blood aeruginosa: metallic help ID bacteria
Blood plate: sheen  Beta - complete lysing of RBCs on plate
(SBA or wounds, Proteus spp.: swarms  Alpha -incomplete or partial lysing.
BAP) eyes, Serratia marcescens: Appears green
throats, brick red  Gamma - non-hemolytic. Colonies are
etc. usually large, white, and raised
Enriched: NAD+ Sterile H. influenzae: needs Grows fastidious organisms
(factor V) and sites, both X and V factors CHOC is SBA that has had RBCs lysed to make
Chocolate hemin (factor X) wounds, H. parainfluenzae: nutrients readily available
(CHOC) eyes, ears, needs factor V
sputums H. ducreyi: needs
factor X
Selective: crystal Wounds, Pseudomonas Inhibits gram pos
violet and bile salts ears, aeruginosa: green- Crystal violet and bile salts allow growth of
MacConke sputums, blue. Considered gram neg bacteria. Inhibits gram pos.
y Differential: lactose urines NLF. Contains
(MAC) pyocyanin (pigment Lactose differentiates between lactose
pH indicator: altering fermenters (LF) and non-lactose fermenters
Neutral Red fermentation) (NLF)
Selective: 7.5% Nasal Staphylococcus Inhibits all gram neg and selects for
NaCl swabs only aureus and Staphylococcus spp.
halophiles (salt- Purpose is to ID S. aureus from other non-
Mannitol Differential: loving organisms) pathogenic Staph spp.
Salt Agar Mannitol
(MSA) NaCl inhibits all gram neg and allows growth
pH indicator: of Staph spp.
Phenol Red Mannitol differentiates mannitol fermenters
(MF) from non-mannitol fermenters (NMF)
MEDIA REFERENCE GUIDE

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