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LAB EXAM 2 all Questions & answers solved 100% accurately with Complete Solution Graded A+ latest version
LAB EXAM 2 all Questions & answers solved 100% accurately with Complete Solution Graded A+ latest version
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LAB EXAM 2✅
oxidase- Positive: BLUE/purple
Negative: brown/yellow
pathway/why: presence of cytochrome oxidase
protocol: filter paper thing DIFFERENTIAL
✅
catalase- Positive: bubble :3
Negative: no bubble boring
Ingredients: drop of peroxide, bubbling means oxygen is being produced
pathway/why: detecting enzyme catalase
protocol: drop peroxide on slide DIFFERENTIAL
✅
starch hydrolysis- Positive: clear zone around line of growth
Negative: no ring around growth
Ingredients: adding iodine, n agar
pathway/why: determine if there is amylase enzyme activity, ability to
hydrolyze starch SELECTIVE and DIFFERENTIAL
✅
gelatin hydrolysis- Positive: gel. broken down —> STAYS LIQUID AFTER
COOLING
Negative: gel. NOT broken —> BACK TO GEL AFTER COOLING
Ingredients: nutrient gel tube; beef extract
pathway/why: ability to produce gelatinases / break down gelatin
protocol: STAB INNOCULATE, DIFFERENTIAL
✅
decarboxylase test- Positive: yellow base (+), PURPLE broth (+)
Negative: yellow base (+), yellow broth (-)
Ingredients: indicator is bromocresol purple, mineral oil (promotes
fermentation of glucose)
pathway/why: to decarboxylase amino acids, DIFFERENTIAL
✅
phenol red fermentation broth- Positive: YELLOW
Negative: red, no ferment
ingredients: gp medium, manitol, lactose, glucose, sucrose; durham tube
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