Exam (elaborations)
DNA and RNA assignment (1).
DNA and RNA assignment (1).
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DNA and RNA assignmentThe following is the nucleotide sequence of a DNA template strand transcribed by RNA
polymerase:
3'- AGG GGA TAC TTC TCT TCC TTA CCC CAT AGG AAA ATC - 5'
What is the sequence of the NON-TEMPLATE DNA strand? (left to right opposites of
the letters) - ANS-5' TCC CCT ATG AAG AGA AGG AAT GGG GTA TCC TTT TAG 3'
The following is the nucleotide sequence of a DNA template strand transcribed by RNA
polymerase:
3'- AGG GGA TAC TTC TCT TCC TTA CCC CAT AGG AAA ATC - 5'
Following transcription, what is the sequence of the mRNA strand? Type in the answer
from 5' --> 3' direction
(SAME AS NON-TEMPLATE just switch T's with U's) - ANS-5' UCC CCU AUG AAG
AGA AGG AAU GGG GUA UCC UUU UAG 3'
Translate the mRNA strand - ANS-Met (Start) Lys Arg Arg Asn Gly Val Ser Phe (Stop)
What is the sequence of the mRNA that does NOT get translated (known as the 5'
untranslated region - 5'UTR)? - ANS-UCC and CUU because the start codon is AUG
(Met)
If the mRNA were from a eukaryotic cell, what special modification would be found at
the 5' end?
at the 3' end? - ANS-5' end= Poly A Tail
3' end= 5' Cap
Suppose a mutation occurs in the original DNA template strand where the fourth T is
mutated to an A. What kind of mutation is this?
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