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MCB4304 Exam 2 Final Questions & Answers 2024/2025
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MCB4304 Exam 2 Final Questions & Answers 2024/2025 When trying to solve a protein structure, why is it necessary to grow protein crystals prior to X-ray-crystallography? - ANSWERSthe proteins must all be oriented in the same manner in the unit cell. The helix-turn-helix DNA binding motif is...

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  • August 6, 2024
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MCB4304 Exam 2 Final Questions &
Answers 2024/2025

When trying to solve a protein structure, why is it necessary to grow protein crystals prior to X-ray-
crystallography? - ANSWERSthe proteins must all be oriented in the same manner in the unit cell.



The helix-turn-helix DNA binding motif is a feature exclusive to prokaryotes. - ANSWERSfalse



Helix-turn-helix DNA binding motifs exclusively interact with DNA base pairs. - ANSWERSfalse



DNA binding proteins can interact with the DNA strand through either the major or minor groove. -
ANSWERStrue



what makes the T4 phage enhancer protein a "weird" case? - ANSWERSIt does not target a specific DNA
sequence, instead it targets the replication fork.



Eukaryotic RNA polymerases are one massive protein structure - ANSWERSfalse



The common subunits are called by this term because they are shared between all eukaryotic RNA
polymerases. - ANSWERStrue



which of the following is NOT part of the core promoter element of class II RNA polymerase genes? -
ANSWERSupstream promoter element



Eukaryotic enhancers and silencers can both be position and orientation independent. - ANSWERStrue



Classical class III prmoter differ from class I and II promoters in what way? - ANSWERSthey lie entirely
within the gene.

, Which of the general transcription factors in class II preinitiation complex formaion is associated with
TATA binding protein? - ANSWERSTFIID



TFIIE and TFIIH are not essential for the formation of an open promoter complex but are required for
promoter clearance in class II genes. - ANSWERStrue



Which general transcription factor complexes with RNA polymerase prior to assembling into the pre-
intitiation complex in class II genes? - ANSWERSTFIIF



Class I genes only have two general transcription factors (plus polymerase) in their preinitiation complex:
a core binding factor (like SL1) and an upstream promoter element binding factor (UBF). - ANSWERStrue



the transcription of all classical class III genes requires TFIIC and TFIIB - ANSWERStrue



What is the importance of x-ray crystallography? - ANSWERSX-ray crystallography shows us what
proteins look like



Light Microscopy - ANSWERSvisible light scattered by an object, then a lens collects the light rays and
focuses on them to create an image.



X-ray diffraction - ANSWERSdiffraction pattern is created when x-ray beams interact with the electron
cloud surrounding atom.



Are any lens capable of focusing X-ray - ANSWERSFalse



Diffraction power of many molecules? - ANSWERSdiffraction power of single molecules is to weak to
detect; many molecules are required



Eukaryotic transcriptional activators have at least a DNA binding domain and a transcription activation
domain - ANSWERStrue

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