100% satisfaction guarantee Immediately available after payment Both online and in PDF No strings attached
logo-home
Previously searched by you
Previously searched by you
logo
MCB3020 Exam 3 Questions and Answers 100% Correct CA$18.84   Add to cart
Quickly navigate to
Preview
  2.  
  3. MCB3020
  4.  
  5. MCB3020

Exam (elaborations)

MCB3020 Exam 3 Questions and Answers 100% Correct
 11 views  0 purchase
  • Course
  • MCB3020
  • Institution
  • MCB3020

MCB3020 Exam 3 Questions and Answers 100% CorrectMCB3020 Exam 3 Questions and Answers 100% CorrectMCB3020 Exam 3 Questions and Answers 100% Correct Reverse transcriptase process - ANSWER - 1. poly-T primer to single-strand RNA 2. reverse transcriptase to produce DNA-RNA hybrid 3. enzyme RNaseH t...

[Show more]

Preview 3 out of 19  pages

Document information

  • August 13, 2024
  • 19
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers

Subjects

  • mcb3020 exam 3 questions and answers 100 correct
  • mcb3020 exam 3 questions and answers stuvia
  • reverse transcriptase process answer 1 poly
  • cloning vector answer allows for transfer of g

Written for

  • MCB3020
  • MCB3020

Seller

avatar-seller
NursingTutor1

Reviews received

Content preview

MCB3020 Exam 3 Questions and
Answers 100% Correct
Reverse transcriptase process - ANSWER - 1. poly-T primer to single-strand RNA
2. reverse transcriptase to produce DNA-RNA hybrid
3. enzyme RNaseH to cut RNA and regenerate RNA primers
4, DNA polymerase and ligase synthesizes new complementary DNA strand


cloning vector - ANSWER - allows for transfer of gene of interest to a host organism; allows for
the making of recombinant DNA


good cloning vector qualities - ANSWER - origin of replication
selectable marker (i.e. antibiotic resistance gene)
unique restriction sites that allow the insertion of gene of interest


types of cloning vectors - ANSWER - plasmid (carry 20k base pairs)
bacteriophage (carry 9k-25k base pairs)
cosmid (carry 30k-47k base pairs)
artificial chromosomes (carry up to 1 million base pairs)


PCR ingredients - ANSWER - primers
Taq polymerase
deoxyribonucleotide triphosphates
interferons - ANSWER - antiviral molecules
alpha, beta, gamma
treats viral infections


primary metabolite - ANSWER - essential for proper growth, its removal significantly impacts
survival

,secondary metabolite - ANSWER - not directly involved in growth; its removal doesn't
significantly impact survival;
productions is usually triggered by exogenous events


protoplast fustion - ANSWER - cells without cell walls are put together in solution, then shocked
electrically to fuse them all together


agricultural applications of genetic manipulations - ANSWER - plant hormones
food additives
genetically modified plants
biopesticides


chemostat - ANSWER - bioreactor with a constant volume caused by a constant inflow of fresh
medium and output of culture liquid


protoplast fustion - ANSWER - cells without cell walls are put together in solution, then shocked
electrically to fuse them all together


agricultural applications of genetic manipulations - ANSWER - plant hormones
food additives
genetically modified plants
biopesticides


chemostat - ANSWER - bioreactor with a constant volume caused by a constant inflow of fresh
medium and output of culture liquid


PCR temperatures - ANSWER - 90 - separates DNA strands
50 - primers attach
70 - nucleotides are added on


types of polymers for gel electrophoresis - ANSWER - agrose

, polyacrylimide


ethidium bromide - ANSWER - stains DNA, makes it visible under UV


RFLP Analysis procedure - ANSWER - 1. isolate gene of interest
2. PCR
3. cut DNA, making restriction fragments
4. gel electrophoresis to separate according to size
5. computers to analyze


ribotyping - ANSWER - compares rRNA to find similarities/differences across domains


southern blotting - ANSWER - 1. cut DNA to make a lot of fragments
2. separate DNA by size via gel electrophoresis
3. place sheet of nitocellulose paper on top of gel
4. place paper in heat-sealed bag with radioactive probe
5. wash filter and place on x-ray film to make autoradiograph


western blotting - ANSWER - detects proteins


northern blotting - ANSWER - detects RNA


making of a genomic library - ANSWER - 1. extract DNA
2. cut into fragments of specific size
3. insert the fragments into a vector
4. insert vector to host organism
5. DNA can now be amplified/retrieved


site-directed mutagenesis - ANSWER - 1. identify sequence
2. complementary DNA sequence in lab with the mutation

The benefits of buying summaries with Stuvia:

Guaranteed quality through customer reviews

Guaranteed quality through customer reviews

Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.

Quick and easy check-out

Quick and easy check-out

You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.

Focus on what matters

Focus on what matters

Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!

Frequently asked questions

What do I get when I buy this document?

You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.

Satisfaction guarantee: how does it work?

Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.

Who am I buying these notes from?

Stuvia is a marketplace, so you are not buying this document from us, but from seller NursingTutor1. Stuvia facilitates payment to the seller.

Will I be stuck with a subscription?

No, you only buy these notes for CA$18.84. You're not tied to anything after your purchase.

Can Stuvia be trusted?

4.6 stars on Google & Trustpilot (+1000 reviews)

75632 documents were sold in the last 30 days

Founded in 2010, the go-to place to buy study notes for 14 years now

Start selling
CA$18.84
  • (0)
  Add to cart