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MCB3020 Exam 1 with Complete Questions and Answers
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MCB3020 Exam 1 with Complete Questions and AnswersMCB3020 Exam 1 with Complete Questions and AnswersMCB3020 Exam 1 with Complete Questions and AnswersMCB3020 Exam 1 with Complete Questions and Answers The proper sequence of reagents in the Gram stain procedure is _____ - ANSWER - crystal violet, i...

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  • August 13, 2024
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  • 2024/2025
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  • mcb3020 exam 1 answers
  • mcb3020 exam 1 with complete questions and answers
  • the proper sequence of reagents in the gram stain

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MCB3020 Exam 1 with Complete
Questions and Answers
The proper sequence of reagents in the Gram stain procedure is _____ - ANSWER - crystal
violet, iodine, ethanol, safranin.


After performing a Gram stain of a mixed culture of Gram-positive and Gram-negative cells, you
realize you forgot to apply the iodine in step 2. What would you expect to see if you observed
the slide under the microscope after this omission? - ANSWER - Pink Gram-positive and pink
Gram-negative cells


The Gram stain of a bacterial cell is based on the chemical properties of its _______. -
ANSWER - cell wall


Acetone can be used as the decolorizer in the Gram stain procedure. - ANSWER - True


The Gram stain can only be performed on cultures grown in liquid media. - ANSWER - False.


Differentiate between the types of microorganisms studied by microbiologists. - ANSWER -
Eukaryote: an organism characterized by having cells containing DNA in a membrane-bound
organelle
Prokaryote: an organism characterized by having a cell lacking a membrane-bound nucleus
Prion: an infectious agent consisting only of protein
Viroid: an infectious agent consisting of only RNA
Virus: an infectious agent made up of protein, nucleic acids, and sometimes a lipid membrane


The most important distinguishing feature of eukaryotes that is lacking in bacteria and archaea
is _____. - ANSWER - the division of the cell into compartments that are bound by membranes.


The instrument that produces a bright image of the specimen against a dark background is
called a(n) ______ microscope. - ANSWER - dark-field

, Gram-positive bacteria have a thinner layer of peptidoglycan than Gram-negative bacteria -
ANSWER - False


Viruses are not generally studied by microbiologists because they are not classified as living
organisms. - ANSWER - False


The earliest microbial fossils that have been found are dated from approximately 4.5 million
years ago. - ANSWER - False


In the following list of scientists who provided the evidence needed to discredit the concept of
spontaneous generation? - ANSWER - Pasteur


Microbiologists study a variety of organisms, but all are considered either Bacteria or Archaea. -
ANSWER - False


In the Gram-staining procedure, the mordant is _____. - ANSWER - iodine


Twitching motility involves _____. - ANSWER - Type IV pili


The major site of cell membrane synthesis is the - ANSWER - endoplasmic reticulum


Confocal microscopes exhibit improved contrast and resolution by - ANSWER - blocking out
stray light with an aperture located above the objective lens.


According to the endosymbiosis hypothesis, - ANSWER - all of the choices are correct.


The relationship between specific bacteria and specific diseases was first demonstrated by
Koch. - ANSWER - True


The cell membrane is a rigid structure that provides bacteria with their characteristic shapes. -
ANSWER - False

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