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Electrophoresis Exam Elaborations – Q’s And A’s
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Electrophoresis Exam Elaborations – Q’s And A’sWhen DNA or RNA is applied to a macromolecular matrix such as agarose or
polyacrylaminde gel Right Ans - electrophoresis
the migration under the pull of current in electrophoresis depends on
Right Ans - 1. size of DNA
2. compact of matrix
3. net charge of molecule
4. shape of molecule
5. electric field strength or voltage drop
6. pore size of gel
7. temperature
the space in the gel Right Ans - compact
___ matrices provide resistance to the movement of molecules under the force
of the electric current Right Ans - gel
matrices prevent ____ and reduce ____ so that the separated molecules form a
defined group Right Ans - diffusions and convention currents
____ must be unaffected by electrophoresis Right Ans - matrices
matrices must be ____ to prepare and ____ to modification Right Ans -
simple; amenable
each nucleotide has a one ____ charge Right Ans - negative
because each nucleotide has a one negative charge, the charge-to-mass ratio
of molecules of different sizes will remain ____ Right Ans - constant
the medium for which electrophoresis is administered Right Ans - agarose
or acrylamide
, refers to the migration of charged molecules through a liquid or gel medium,
agarose or acrylamide, under the influence of an electric field Right Ans -
electrophoresis
DNA is ____ charged at a neutral pH Right Ans - negatively
why is DNA negatively charged Right Ans - phosphate backbone
by introducing a electrical current, migration of DNA will occur towards the
_____ Right Ans - anode
the positive pole Right Ans - anode
the negative pole Right Ans - cathode
nucleic acids are negatively charged and migrate towards the _____ pole
Right Ans - positive/ anode
DNA fragments will migrate at speeds inversely related to their ____ Right
Ans - size
electrophoresis can have a _____ format Right Ans - horizontal or vertical
electrophoresis can be performed in ____ Right Ans - tubes, slab gels, or
capillaries
you would want to put smaller molecules in a ___% agarose gel Right Ans -
2.0
why would you put smaller sized base pairs in a large percent agarose gel
Right Ans - so they don't move all the way to the bottom of the gel
the more electrical charge that is added to the gel the ___ the particles will
move Right Ans - faster
the agarose gel is designed to ____ movement of particles through the matrix
Right Ans - slow
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