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PTC Lab Comprehensive Exam Questions And Complete Detailed Answers.
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Purpose of this lab - correct answer Analyze our own DNA to see what form of TAS2R38 (taster gene): heterozygous, homozygous (dominant or recessive) What did we use to extract DNA from us? - correct answer ...

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  • August 16, 2024
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  • 2024/2025
  • Exam (elaborations)
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PTC Lab


Purpose of this lab - correct answer Analyze our own DNA to see what form of
TAS2R38 (taster gene): heterozygous, homozygous (dominant or recessive)



What did we use to extract DNA from us? - correct answer Saline solution



Importance of saline solution - correct answer Salt neutralizes the negative
charge of the sugar phosphate backbone of DNA --> causes DNA to become less hydrophilic



Scraped away cells from mouth



Centrifuge - correct answer device to separate substances by spinning them at
high speed



What happens when we spun the centrifuge after using the saline solution? - correct answer
makes the heavier molecules move to the bottom of the test tube; pushed cells to the bottom which
formed a pellet (will be used for DNA extraction)



After centrifuging the 1st time, what did we do - correct answer added lysis
buffer (red) to resuspend the cell



lysis buffer - correct answer solution (which had proteinase K) used to break
the cell membrane and release cell contents (DNA)



why did we do a water bath for 55°C? - correct answer incubate the cell
solution during cell lysis;



- Optimal temperature for the enzyme Proteinase K to actively destroy cellular machinery.

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