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GMS6121 Exam 3 Questions with Answers

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GMS6121 Exam 3 Questions with Answers Electron microscopy - Answer-Allows visualization of virus particles in lesions Laborious, but easy to look at Immunofluorescence - Answer-Uses virus-specific antibody to detect viral protein Usually in tissue biopsy or cells from fluid Easy, gold...

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  • August 20, 2024
  • 13
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers

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  • electron microscopy
  • gms6121 exam 3 questions with answers

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GMS6121 Exam 3 Questions with
Answers

Electron microscopy - Answer-Allows visualization of virus particles in lesions

Laborious, but easy to look at

Immunofluorescence - Answer-Uses virus-specific antibody to detect viral protein

Usually in tissue biopsy or cells from fluid

Easy, gold standard

ELISA - Answer-Uses virus-specific antibody to detect virus particles or proteins in fluid

Lateral flow assay - Answer-Rapid ELISA essentially

If analyte is present, it complexes with labeled antibody. The complex flows up the
membrane and complexes with the primary antibody (test line) and secondary antibody
(control line)

qPCR and RT-qPCR - Answer-Used to quantify number of copies of virus genomes

qPCR = DNA
RT-qPCR = RNA, uses reverse transcriptase

Serology - Answer-Indirect detection of virus

Detects antibodies to a virus in serum

Does not necessarily indicate active infection

Often measured by ELISA
Sometimes measured by neutralization or hemagglutination inhibition

Diagnostics methods depend on ________ - Answer-Suspected virus
Clinical symptoms
Available tests

Syndromic testing - Answer-Use of multiplex panels

, Ex: BIoFire FilmArray GI Panel

Cellular outcomes of viral infection - Answer-Cell death
Abortive infection
Persistent infection
Transformation

Cell death/CPE due to infection - Answer-Due to:
Diversion of cell's energy
Shutoff of synthesis
Competition for ribosomes and TFs

Results in pyrogens: Fever inducing

Abortive infection - Answer-Virus initiates infection but does not generate infectious
particles

Due to:
Lack of replication conditions
Host antivirals

Cell may be damaged, killed, or transformed, but does not release infectious progeny

Persistent infection - Answer-No cell death or alteration

1) Chronic
2) Latent
3) Recurrent

Chronic infection - Answer-Constant production of virus at low levels
Long incubation period

Ex: HBV, HCV

Latent infection - Answer-No production of viral progeny, but genome is maintained

Low-level reactivation

Ex: Herpesviruses

Recurrent infection - Answer-Reactivation of a latent infection due to stress or other
factors

Ex: HSV

Transformation - Answer-Virus alters cell

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