Exam (elaborations)
MT AAB IMMUNOHEMATOLOGY EXAM QUESTIONS WITH CORRECT ANSWERS
- Course
- Institution
MT AAB IMMUNOHEMATOLOGY EXAM QUESTIONS WITH CORRECT ANSWERS
[Show more]
Seller
Follow
biggdreamer
Reviews received
Content preview
MT AAB STUDY GUIDE TESTQUESTIONS WITH CORRECT ANSWERS
What term describes the morphology of organisms that vary in shape and size? -
Answer-Pleomorphic
i.e. coccobacilli
Bacteriology arrangements: - Answer--pairs (diplococci "N. Gon"; diplobacilli
-chains (streptococci, streptobacilli)
-grape like clusters (staphylococci)
-group of four-tetrads (peptococcus)
-packets of eight-cuboidal (sarcina)
-palisades (corneybacterium)
Capsules are the basis of which test? - Answer-serotyping
and identification (capsules are antigenic); they are observed using India ink (negative
staining)
representative organisms (Strep pneumo, Kleb pneumo, Haemophilus influenza, and
Neisseria meningitidius)
A mucoid colony on blood agar medium indicates that which of the following
characteristics may be present? - Answer-Organism has a capsule.
-it surrounds some bacteria
What are dark staining granules called? - Answer-Metachromatic granunles
-affinity for basic dyes
-stains such as Albert, Neisser, and methylene blue
Which of the following organisms are non-motile? - Answer-Shigella and Klebsiella
(with exception to Salmonella--other "ella" are nonmotile)
Shigella (neg) vs Salmonella (pos)
Klebsiella (neg) vs Enterobacter (pos)
Bacillus anthracis (neg) vs most bacillus species (pos) best observed at 25C
35-37C ,au be inhibitory
In the bacterial growth cycle, growth ceases because nutrients are exhausted or toxic
metabolic products have accumulated in which phase? - Answer-Stationary phase
,(plateau ceases-nutrients exhausted/toxic)
Which of the following genera of bacteria form spores? - Answer-Bacillus and
Clostridium
endospores-non feeding, non motile, non reproductive
-highly resistant to heat, drying, freezing; toxic chemical & radiation.
(Sporulating bacteria-bacteria in which spores are forming)
What term is used to describe bacteria that grow in the absence of atmospheric oxygen
and obtain oxygen from oxygen-containing compounds? - Answer-Anaerobes
Aerobes - Answer-grows in presence of (free) O2
Capnophiles - Answer-growth enhanced by increased CO2
aerotolerants - Answer-does not grow well but tolerate (survive) in presence of oxygen
What is the temperature at which bacteria grow best known as? - Answer-Optimum
mesophilic - Answer-grows best 35-37C; most organisms will grow at 20C
thermophilic - Answer-heat loving (50*55C); autoclaving procedures
psychotrophilic - Answer-cold loving (10C)-blood bank contaminancts
On blood agar plates a small zone of alpha hemolysis surrounded by a zone of beta
hemolysis after refrigeration is known as what type of hemolysis? - Answer-Alpha-prime
hemolysis
-Beta hemolysis (clear, colorless-complete hemolysis)
-alpa hemolysis (green, incomplete hemolysis)
-Gamma hemolysis (no hemolysis)
What is the most effective method of sterilization? - Answer-Autoclaving (steam at 121C
@ 16 lbs pressure for 15-30 min)-**QC Bacillus STEAROTHERMOPHILUS
-boiling method (kills all veg-not all spores/viruses (100C for 15-30 min)
Under Fractional Sterilization (used to sterilize media containing mile or serum)
-alternate heating which kills vegetative cells, followed by incubation when spores
germinate, followed by heating, which kills the remaining
tyndallization-flowing steam for 30 min on 3 successive days
-inspissation-75-80C for 2 hrs on 3 successive days
,Which of the following is used for quality control for the dry heat oven? - Answer-
Bacillus subtilis
Dry Heat method
-kills by oxidation
-oven 160-180C for 1-2 hours
-QUALITY CONTROLS (Bacillus Subtilis)
Media that cannot be heated can be sterilized by what alternate method? - Answer-
Filtration
asbestos filter-98% effective
membrane filter-0.22 micron = 100% bacterial sterility
for media that are degraded by heat;
Which one of the following disinfectants is the most effective germicide against bacteria,
spores, tubercle bacilli, fungi, and viruses? - Answer-Chlorine (bleach)
oxidizes organic matter
2% aqueous glutaraldehyde
safe for delicate lensed instruments
poor activity against Pseudomonas
What is a term that describes a process or treatment that renders a medical device,
instrument, or environmental surface safe to handle? - Answer-Decontamination
process can range from sterilization or disinfection to just cleaning with soap and water
What color do gram-positive bacteria stain? - Answer-Purple
- Crystal violet (1 min) will stain for gram positive
- Mordent iodine (1 min) increases affinity or binding of dye
- Decolorizer has 95% alcoholic or aqueous sol'n of dye (2-5 seconds)--most critical
step
- safranin (counterstain) (1 min) will stain for gram neg bact
Acid-fast bacteria appear what color microscopically? - Answer-Red
Hot: Ziehl-Neelson retains the primary dye (carbohlfuchsin)
Acid-fast organisms in tissue are best stained by which of the following methods? -
Answer-Kinyoun
Cold: Kinyoun also stains acid fast organisms in TISSUE
, Which of the following should be used to remove antimicrobials before culturing? -
Answer-Antimicrobial removal device (ARD)
this is for patients taking antibiotics, can utilize a special broth to remove the antibiotics
prior to culture.
Which statement concerning sodium polyanethol sulfonate (SPS) is NOT true? -
Answer-Activates some antimicrobials
What are some functions of sodium polyanethol sulfonate (SPS)? - Answer-Prevents
blood from clotting, prevents phagocytosis, and neutralizes the bactericidal effect of
human serum.
What is the most abundant normal flora in throat cultures? - Answer-Alpha-hemolytic
streptococci
Why is it recommended to stab the blood agar plate when setting up throat cultures? -
Answer-Some streptococci may not produce beta hemolysis under aerobic conditions.
culture may include anaerobic conditions for Beta Strept
What is the most common pathogen is throat cultures? - Answer-Streptococcus
pyogenes
Nasopharangeal swabs are recommended for the detection of which carriers? - Answer-
H. influenzae, N. meningitidis, and B. pertussis
Why is a first morning urine specimen preferred for urine cultures? - Answer-Specimen
is more concentrated.
What indicates vaginal or urethral contamination of urine? - Answer-Greater than 10
squamous epithelial cells per hpf.
How are smears of cerebrospinal fluid prepared? - Answer-Centrifuged CSF sediment
Smears of CSF are usually stained with which stain? - Answer-Gram stain
or India ink (cryptococcus)
Which of the following tests for bacterial antigen on cultures isolated from CSF is more
sensitive and faster? - Answer-Latex agglutination tests
Which of the following is not the cause of a venereal disease? - Answer-Haemophilus
aegyptius
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller biggdreamer. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for CA$35.07. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
79271 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now