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BCH210 TESTED QUESTIONS WITH REVISED ANSWERS – UPDATED CA$18.42   Add to cart

Exam (elaborations)

BCH210 TESTED QUESTIONS WITH REVISED ANSWERS – UPDATED

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BCH210 TESTED QUESTIONS WITH REVISED ANSWERS – UPDATED cross linker can be used in what techniques - Answer-stabilize X ray structure limitation of BiOID - Answer-can only proximate, maybe the proteins are just passing by the POI beads connect to what in BiOid - Answer-biotin released by...

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  • August 30, 2024
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  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • BCH210
  • BCH210
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BCH210 TESTED QUESTIONS
WITH REVISED ANSWERS –
UPDATED

cross linker can be used in what techniques - Answer-stabilize X ray structure

limitation of BiOID - Answer-can only proximate, maybe the proteins are just passing by
the POI

beads connect to what in BiOid - Answer-biotin released by ligase

what is PDI - Answer-protein disulfide isomerase
a kind of chaperon, isomerase

structure of DE at pKa, why - Answer-C=O no - charge because protonated

are cofactors proteins - Answer-no

what is heme - Answer-prothetic organic cofactors, bind tightly, shuttle O2

why N S are beta turn AA - Answer-their R group can be modified for PTM

describe zinc finger - Answer-is a motif, zinc is the ligand, bind 2 cys and 2 his, for
stabilizing and DNA recognition

chaperon binds to where and has what property - Answer-hydrophobic, amphipathic

two sites of enzyme - Answer-active-binds substrate
allosteric- binds to other for conformational change

basic and acid are hydrophilic - Answer-si

imidazole is an aromatic ring - Answer-si

mass spectrometry can only be used on where - Answer-known peptides
Dialysis prefer what proteins - Answer-large

affinity chromatography, what is the bond between resin and ligand, ligand and POI -
Answer-covalent noncovalent

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