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ACS Biochemistry 1st Semester Exam 2024 Questions and Verified Answers, 100% Guarantee Pass
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ACS Biochemistry First Semester.pdf file:///C:/Users/HP/Desktop/New%20folder/ACS%20Biochemistry%20ACS Biochemistry First Semester
1. Buffer Order and Henderson Hasselbach: Water, Buffer, Salt, BasepH = pKa +
log(A/HA)
pKa decrease as temp increase so pH decrease
2. Ramachandran plots: ¦ =N-RÈ
=C-R
optimal angle to prevent steric hinderance
3. Protein structure of a helix, B sheet, reverse turn: a: LEMARKHQB:
VICYFTW
RT: PSDNG
4. how many amino acids in alpha turn helix: 3.6
5. Super secondary structure: helix turn helix
6. parallel vs. antiparallel: parallel wedges face same way
antiparallel face opposite
7. Isoelectric point (pI): pH at which a particular molecule carries no net electricalcharge.
8. Protein Separation Techniques (ALL): HomogenizationSalting
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Out
Affinity Chromatgraphy
Ion Exchange
Size Exclusion/Gel FiltrationH,
S, A, I, SG
He said all individuals SinG
9. Protein Separation Techniques (H): Homogenizationdisrupt
cells, sonicator, centrifuge
10. Protein Separation Techniques (S): Salting out
different proteins precipitate out at different salt concentrations
11. Protein Separation Techniques (A): Affinity chromatography
add a tag; histidine binds; see how well there is an affinity for the group
12. Protein Separation Techniques (I): ion exchange
separation based on charge; + proteins stick to - beads
13. Protein Separation Techniques (SG): Size Exclusion/Gel Filtrationseparation
based on size
14. Cuts (protein separation techniques): cut out what dont need/isnt protein(use 40%
concen. then keep liquid; if use 60% concen. then keep pellet)
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15. Dialysis: protein separation based on bad with pores
1. Moles in bag + moles in buffer
2. Divide moles by total volume - new concentration
16. Electrophoresis: gels - move by size with electric current
1. Isoelectric focusing - purely pH
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