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BIOD 171 Module 4 Quiz with Verified Answers |Latest 2024/2025
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What are the requirements of a fastidious microbe? A fastidious microbe is an organism with complex growth requirements and will not grow without then. Enriched medias thus contain these specific and essential nutrients required for the growth of a particular subset of microorganisms. What is ...

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BIOD 171 Module 4 Quiz with Verified
Answers.

What are the requirements of a fastidious microbe?
_;- _;- _;- A fastidious _;- _;- _;- _;- _;-_;- _;- _;- _;-


microbe is an organism with complex growth requirements and will not
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-


grow without then. Enriched medias thus contain these specific and
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;-


essential nutrients required for the growth of a particular subset of
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-


microorganisms.



What is the name of the process of spreading a bacterial culture onto a
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-


petri dish?
_;- Plating. Plating microbes can be done using a sterile
_;-_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-


loop, a sterile swab, or a sterilized wire loop. Each device is simply a
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-


means of spreading the bacteria, most commonly in a simple back-and-
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;-


forth motion, across the plate.
_;- _;- _;- _;-




What is the primary advantage of plating a bacterial culture as opposed
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-


to having it grow in solution?
_;- The primary advantage of plating a
_;- _;- _;- _;- _;-_;- _;- _;- _;- _;- _;- _;- _;-



bacterial sample onto agar is that cells are held in place. Unlike in a
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-


nutrient broth where bacterial cells can multiply but are free to move
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-



around in solution, bacteria plated onto agar are fixed in such a way as
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-


to support the formation and visualization of colonies.
_;- _;- _;- _;- _;- _;- _;-




What is the purpose of the quadrant streak approach?
_;- _;- _;- The purpose _;- _;- _;- _;- _;- _;-_;- _;- _;- _;-


of this method is to generate an individual colony so that a single (pure)
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-


bacterial sample can be picked from the plate.
_;- _;- _;- _;- _;- _;- _;-

,What are the requirements of a fastidious microbe?
_;- _;- A fastidious
_;- _;- _;- _;- _;- _;-_;- _;- _;- _;-



microbe is an organism with complex growth requirements such that if
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-


absent it will not grow. Enriched medias thus contain these specific and
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-



essential nutrients required for the growth of a particular subset of
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-


microorganisms.



What is agar? _;-In its simplest form agar is liquid growth media that
_;- _;-_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-



has had a hardening agent added to it. Agar is created when a
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-


polysaccharide derived from seaweed (algae) extract is added to growth
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;-


media. Agar is used to create a solid, smooth surface on which microbes
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-


can grow.
_;-




LB agar is classified as a non-selective, differential media
_;- _;- _;- _;- _;- _;- _;- _;-




True
False _;-_;- _;- False



LB agar is non-selective and non-differential
_;- _;- _;- _;- _;-




What are the three forms of hemolysis found on blood agar plates?
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-_;- _;-



Alpha hemolysis, beta hemolysis, and gamma hemolysis
_;- _;- _;- _;- _;- _;-




Alpha hemolysis _;- Presents with greenish-brown color on blood agar _;-_;- _;- _;- _;- _;- _;- _;- _;- _;-


due to the partial lysis of red blood cells
_;- _;- _;- _;- _;- _;- _;- _;-

, Beta hemolysis
_;- Clear zones on blood agar indicate beta hemolysis, _;-_;- _;- _;- _;- _;- _;- _;- _;- _;- _;-


which is the complete lysis of red blood cells
_;- _;- _;- _;- _;- _;- _;- _;-




Gamma hemolysis Presents a white/tan on blood agar due to the
_;- _;-_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-


lack of hemolytic activity.
_;- _;- _;-




What is the primary purpose of Columbia CNA agar?
_;- _;- _;- As it _;- _;- _;- _;- _;- _;-_;- _;- _;- _;-



suppresses the growth of Gram-negative bacteria CNA agar is, therefore,
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;-


used for isolation of Gram-positive microbes.
_;- _;- _;- _;- _;-




Name the type of plate derived from BAP that contains lysed red blood
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-


cells Lysed red blood cells (RBCs) are a primary component in the
_;-_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-


formulation of Chocolate agar. _;- _;- _;-




Define the selective and differential abilities of a MacConkey agar plate
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-_;-


MacConkey agar is selective in that only Gram-negative microbes
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;-


will grow on the agar -Gram-positive microbes simply do not grow.
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-


MacConkey agar is also considered a differential media as it
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;-



distinguishes between microbes capable of fermenting lactose (red
_;- _;- _;- _;- _;- _;- _;- _;-


colonies) and those that are non-fermenters (white/tan colonies).
_;- _;- _;- _;- _;- _;- _;-




What colors would you expect to see on an EMB plate containing E.
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-



coli? The EMB plate itself is red in color while in the presence of E.
_;-_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-


coli, the growing colonies will take on a distinctive metallic green sheen.
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

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