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BIOL 203 MOLECULAR BIOLOGY LAB 12 REPORT HUNTER COLLEGE
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lOMoAR cPSD| 42147428BIOL 203 MOLECULAR BIOLOGY
LAB 12 REPORT HUNTER
COLLEGE
, lOMoAR cPSD| 42147428
BIOL203- SPRING
2023/2024
LAB12 LAB REPORT
Exercise 1. Homology searching using BLAST
1. Species and strain: Mus musculus strain C57BL/6J (House
mouse)
2. Chromosome: 10
3. Length of your query sequence: 411
4. Percent identity, number of matched bases, and number of gaps between
the matched sequences: 99% identity match, 402/403 bases matched,
0/403 gaps between the matched sequences.
1. Locus ID: NC_000076
2. Total length of the gene: 21884 bp
3. Number of exons: There are 11 exons.
4. How many mRNAs do you find for this sequence? How many exons are
there for the smallest mRNA? Why are there several mRNAs shown for
one gene?
There are 4 mRNAs for this sequence. There are 7 exons in the smallest
mRNA. There are multiple mRNA for 1 gene because each mRNA codes
for different proteins which express the gene.
Exercise 2. Explore the structure of human mdm2 gene
Gene name: MDM2 proto-oncogene
Brief description of its function: Promotes tumor formation by targeting tumor
suppressor proteins like p53, resulting in proteasomal degradation.
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