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Chapter 23 Abscisic Acid Questions and Answers
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Chapter 23 Abscisic Acid Questions and Answers Location of abscisic acid present in all parts of plant Bioassays stomatal closure; inhibition of germination, coleoptile growth, and amylase synthesis in seeds; various gene expression assays-- physical methods are better, though (immunoassa...

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  • September 28, 2024
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  • 2024/2025
  • Exam (elaborations)
  • Questions & answers

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  • chapter 23 abscisic acid questions and answers
  • location of abscisic acid present in all parts of
  • bioassays stomatal closure inhibition of germinat

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Chapter 23 Abscisic Acid Questions and
Answers
Location of abscisic acid - answer present in all parts of plant

Bioassays - answer stomatal closure; inhibition of germination, coleoptile growth,
and amylase synthesis in seeds; various gene expression assays-- physical methods
are better, though (immunoassays, gas chromatography if the ABA is purified first,
HPLC)

Basic facts about abscisic acid - answer synthesized in all cells with plastids;
synthesized from a carotenoid intermediate (via terpenoid pathway); "growth inhibitor",
(increases dormancy); "stress hormone" (especially water stress, which leads to
stomata closure)

Abscisic acid is transported in... - answer vascular tissue, especially phloem

First effect of ABA - answer Induces dormancy of seeds and buds (or quiescence)

Second effect of ABA - answer Increases desiccation tolerance of embryo (via
protective proteins) and increases storage proteins

Third effect of ABA - answer Stomatal closure (ABA synthesized in roots in drying
soil, results in signal to decrease transpiration)

Fourth effect of ABA - answer senescence of leaves (independently of ethene)

Fifth effect of ABA - answer increased root growth (a lot, via suppressing ethene in
root) and decreased shoot growth (a bit) IF plant is water stressed (leads to an
increased root-to-shoot biomass ratio)

Sixth effect of ABA - answer High [ABA] leads to an increase in the production of
ethene

ABA redistribution is affected by... - answer pH; anion trap concept: ABA gets
trapped in alkaline (higher-pH) compartments because -> ABA- (not ABA-H) (recall: ions
cannot diffuse across membranes)

Water stress - answer -> increased pH of xylem sap (more alkaline); -> increased pH
of apoplast of mesophyll; -> increased [ABA-] in apoplast and xylem

Dehydration - answer -> ↓ pH of cytosol in the mesophyll (acidic); -> increased [ABA-
H] escaping from cytosol

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