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SLCC Microbiology Lab Final Review Exam Questions and Answers
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SLCC Microbiology Lab Final ReviewExam Questions and Answers
Blood iiagar ii- iiAnswers ii-nutrient iienriched iimedia iiused iito iiidentify iihemolytic
iienzymatic iiorganisms
Phenylethyl iiAlcohol ii(PEA) ii- iiAnswers ii-Selective: iiThis iimedium iiis iiused iifor iithe
iiisolation iiof iithe iimost iigram-positive iiorganisms. iiIt iiis iipartially iiinhibitory iito iigram-
negative iiorganisms iiwhich iimay iiform iivisible iicolonies iiwhose iisize iiand iinumber iiare
iimuch iismaller iithan iion iiother iimedia
Mannitol iiSalt iiAgar ii(MSA) ii- iiAnswers ii-Selective/differential: iiThis iimedium iiis
iiinhibitory iito iimost iiorganisms iiother iithen iithe iihalophilic ii(salt iiloving) iimicroorganisms.
iiIsolate iiorganisms iithat iican iiferment iiMannitol
MacConkey iiAgar ii- iiAnswers ii-Selective iitest: iifor iigram iinegative iibecause iithe iicrystal
iiviolet-which iiinhibits iigram-postitive. iiBile iisalts iiselects iifor iienteric iibacteria
Differential: iifor iilactose iifermentation iiRed/pink
Coliform iiBacilli ii- iiAnswers ii-will iiferment iishowing iired/hot iipink-normal
Dysentery, iityphoid, iiparatyphoid iibacilli ii- iiAnswers ii-grow iion iiMAC iidon't iiferment
iilactose ii
The iibacteria iicolonies=uncolored/transparent
Eosin-Methylene iiBlue iiAgar ii- iiAnswers ii-Selective: iifor iigram iinegative iidue iipartially
iiinhibitory iiof iigram iipositive ii
Differential iifor iifermentation iilactose/sucrose:
Vigorous: iiAcid iipH iiindicator iiappears iiblue/black iiwith iigreen iimetallic iisheen
Slow: iiappears iipink/purple iimucoid
Non: iiproduce iino iiacid iiappears iicolorless/transparent
Embden-Meyerhof iiPathway ii- iiAnswers ii-the iiglycolytic iipathway iiwhen iiglucose iiis
iimetabolized iiinto ii2 iipyruvic iiacids
Catalase iiTest ii- iiAnswers ii-if iithe iiorganism iihave iicatalase iiit iiwill iiproduce iibubbles
iiwhen iihydrogen iiperoxide iiis iiadded iiH₂O₂→(Catalase) ii2H₂O ii+O₂
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