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MCB 2610 Lab final Question and answers already passed 2024/2025
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MCB 2610 Lab final Question and answers already passed 2024/2025 MCB 2610 Lab final The ______ _______ method is a quick and easy way to isolate bacteria - correct answer streak plate In what orientation do you incubate the plates after they have been streaked? - correct answer agar ha...

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  • October 17, 2024
  • 13
  • 2024/2025
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  • Questions & answers

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MCB 2610 Lab final
The ______ _______ method is a quick and easy way to isolate bacteria -
correct answer ✔streak plate


In what orientation do you incubate the plates after they have been streaked?
- correct answer ✔agar half bottom


What must you do to the loop before every use? - correct answer ✔flame it


T/F: The last thing you do before leaving the lab is take off your lab coat -
correct answer ✔false


streaked plate method - correct answer ✔culture is spread out on agar to
make isolated colonies


solid media (agar plates) - correct answer ✔used to isolate bacteria


liquid culture media (broth) - correct answer ✔used to grow cells to high
densities


T/F: When making a smear of the culture, you heat fix the slide before air
drying it completely - correct answer ✔false


What is the magnification value of the ONLY objective that should come in
contact with immersion oil? - correct answer ✔100x

, Pertaining to the Gram stain, cells that retain the primary stain and appear
purple are said to be Gram - correct answer ✔positive


What cellular structure does the counterstain safranin stain? - correct answer
✔outer membrane


direct stain - correct answer ✔stains the organism itself


indirect or negative stain - correct answer ✔stains the background


simple stain - correct answer ✔use only one stain to determine cell
morphology, size and arrangement


Gram staining stains the cell __________, while spore staining stains for
__________. - correct answer ✔cell envelope, endospores


Gram Positive cell characteristics - correct answer ✔thick peptidoglycan
layer, retains crystal violet, no outer membrane


Gram Negative cell characteristics - correct answer ✔thin layer of
peptidoglycan, doesn't retain crystal violet, has outer membrane, safranin
counterstains membrane pink


Gram stain steps and time - correct answer ✔1. crystal violet, 1 minute, 2.
iodine, 1 minute, 3. ethanol, 15-30 seconds, 4. safranin, 30 seconds


Why do bacteria form endospores? - correct answer ✔under environmental
stress

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