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MCB 2050 Molecular Biology of the Cell upcoming exam 2024 cheat sheet with Prof. K. Yankulov University of Guelph CA$18.14   Add to cart

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MCB 2050 Molecular Biology of the Cell upcoming exam 2024 cheat sheet with Prof. K. Yankulov University of Guelph

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  • MCB 2050 Molecular Biology of the Cell
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  • MCB 2050 Molecular Biology Of The Cell

MCB 2050 Molecular Biology of the Cell upcoming exam 2024 cheat sheet with Prof. K. Yankulov University of Guelph I synthesize pre-mRNA, what polymerase am I? a) DNA polymerase b) RNA pol III c) RNA pol II d) RNA pol I d) RNA pol I __________ is darkly staining and densely packed chrom...

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  • October 18, 2024
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  • 2024/2025
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  • MCB 2050 Molecular Biology of the Cell
  • MCB 2050 Molecular Biology of the Cell
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MCB 2050 Molecular Biology of the Cell upcoming exam
2024 cheat sheet with Prof. K. Yankulov University of
Guelph




I synthesize pre-mRNA, what polymerase am I?

a) DNA polymerase

b) RNA pol III

c) RNA pol II

d) RNA pol I

d) RNA pol I




__________ is darkly staining and densely packed chromatin which is transcriptionally __________.

a) euchromatin; inactive

b) euchromatin; active

c) heterochromatin; inactive

d) heterochromatin; active

c) heterochromatin; inactive




Where is the core promoter relative to the +1 site of a gene?

a) Elements of the core promoter are located within about 35 base pairs both upstream and
downstream of the +1 site

,b) The location of the core promoter is not related to the +1 site

c) It can be hundreds of base pairs upstream or downstream from the +1 site

d) The core promoter is centred at ~-30 relative to the +1 site

e) The core promoter is located several base pairs downstream of the +1 site

a) Elements of the core promoter are located within about 35 base pairs both upstream and
downstream of the +1 site




What promoter element would you expect to find upstream of a gene that is highly expressed?

a) Initiator box

b) CpG islands

c) TATA box

d) DPE

c) TATA box




What promoter element would you expect to find upstream of a gene that is poorly expressed?

a) BRE

b) CpG islands

c) TATA box

d) DPE

b) CpG islands




A subunit within the general transcription factor __________ binds the TATA box during transcriptional
activation.

,TFIID




Put the following steps in order:

_____ A preformed complex of TFIIF and RNA pol II bind

_____ The TBP subunit of TFIID binds the TATA box, bending DNA

_____ TFIIA and TFIIB bind

_____ TFIIE binds creating a docking site for TFIIH, which then binds

1. The TBP subunit of TFIID binds the TATA box, bending DNA

2. TFIIA and TFIIB bind

3. A preformed complex of TFIIF and RNA pol II bind

4. TFIIE binds creating a docking site for TFIIH, which then binds




RNA pol II and the general transcription factor __________ bind the promoter as a pre-formed complex

TFIIF




In order to use a promoter plasmid to study the regulatory elements of a gene of interest, the
_________ of your gene of interest is inserted into the reporter plasmid _________.

a) open reading frame; upstream of the reporter gene

b) exons; upstream of the plasmid reporter

c) coding region; downstream of the plasmid reporter

d) promoter region; upstream of the reporter gene

e) structural region; downstream of the reporter gene

d) promoter region; upstream of the reporter gene

, Which element within the eukaryotic promoter has the most significant impact of transcription when
deleted?

Relative promoter activity after deletion....

TATA box- 38%

-40 to -70 region- 95%

GC box- 14%

CAAT box- 50%

-110 to -130 region- 114%

GC box




Sex determination in Drosophila embryos and post transcriptional regulation of the sxl, tra, and dsx
transcripts are an example of:

a) Non-disjunction

b) Alternate splicing

c) Gene expression

d) Transcriptional regulation

b) Alternate splicing




Which of the following RNA molecule binds perfectly to mRNA leading to it's rapid degradation?

Question 2 options:

a) miRNA

b) siRNA

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