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Brock Biology of Microorganisms - Chapter 9 Exam Study Guide. CA$15.08   Add to cart

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Brock Biology of Microorganisms - Chapter 9 Exam Study Guide.

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Brock Biology of Microorganisms - Chapter 9 Exam Study Guide. Genome - answerentire complement of genetic information (including genes, regulatory sequences, and noncoding DNA) genomics - answerdiscipline of mapping, sequencing, analyzing, and comparing genomes sequencing - answerdetermining t...

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  • October 18, 2024
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  • Brock Biology of Microorganisms
  • Brock Biology of Microorganisms
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©SIRJOEL EXAM SOLUTIONS
10/3/2024 9:54 PM


Brock Biology of Microorganisms - Chapter
9 Exam Study Guide.



Genome - answer✔entire complement of genetic information (including genes, regulatory
sequences, and noncoding DNA)

genomics - answer✔discipline of mapping, sequencing, analyzing, and comparing genomes

sequencing - answer✔determining the precise order of nucleotides in a DNA or RNA molecule

genome annotation - answer✔converting raw sequence data into a list of genes present in the
genome ("bottleneck")

bioinformatics - answer✔storing and analyzing sequences and structures

dideoxy method - answer✔-method of sequencing DNA invented by Fred Sanger
-copy original ssDNA
-small amounts of dideoxyribonucleotides (ddNTPs) used with dNTPs
-originally used radioactivity, but now use fluorescent labels

dideoxyribonucleotides (ddNTPs) - answer✔chain terminators that prevent further elongation of
DNA chain

pyrosequencing - answer✔-"next-generation sequencing"
-uses luciferase (bioluminescent enzyme) to detect incorporation of dNTPs

Functional ORF - answer✔ORF that actually encodes a protein; can be identified by a computer

codon bias - answer✔some codons used more frequently than others

how to find and identify ORFs - answer✔1) locate start and stop codons and Shine-
Dalgarno/ribosome-binding sequences

, ©SIRJOEL EXAM SOLUTIONS
10/3/2024 9:54 PM

2) look for similarity to ORFs in other genomes

hypothetical proteins - answer✔-uncharacterized ORFs; proteins that likely exist but whose
function is currently unknown (due to lack of aa sequence homology with knowns)
-assigned to family or general function

noncoding RNA - answer✔-RNA that is not translated (like tRNA, rRNA, noncoding regulatory
RNAs)
-lack start codons and have multiple stop codons

comparative genomics - answer✔uses databases to probe genomes for correlation between
genome size and ORF content

small genomes - answer✔-these genomes belong to parasitic or endosymbiotic prokaryotes
-this size means these symbionts are totally dependent on host for nutrients, but provide host
with essential aa and nutrients it can't synthesize
-Ex. Mycoplasma (Bacteria) and Nanoarchaeum equitans (Archaea) are the most extreme of
these
-probably needs at least 250-300 genes to be viable
-tend to encode more translational processes

large genomes - answer✔-some bacterial genomes are as "***" as some eukaryotic microbes
-can even have more genes than eukaryotes due to lack of introns
-Ex. Sorangium cellulosum (Bacteria)
-Archaeal extremes are typically smaller than Bacteria extremes in this manner
-tend to encode more transcriptional regulation and signal transduction processes

metabolic genes - answer✔typically the most abundant class of genes

Archaeal genomes - answer✔-these genomes tend to devote higher % of genomes to energy and
coenzyme production
-have fewer genes for carbohydrate metabolism and membrane functions
-subject to change as more types of this genome are able to be studied

mitochondria and chloroplast genomes - answer✔-encode necessary machinery for protein
synthesis

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